USP25 抗体 (AA 1-1053)
Quick Overview for USP25 抗体 (AA 1-1053) (ABIN7598940)
抗原
See all USP25 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-1053
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原理
- Anti-USP25 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-USP25 Antibody Picoband® (ABIN7598940). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human USP25 recombinant protein (Position: M1-D1053).
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亚型
- IgG
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anti-Ubiquitin Specific Peptidase 25 (USP25) antibody
USP25 适用: 人, 大鼠, 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin Specific Peptidase 25 (USP25) (AA 348-606), (C-Term) antibodyUSP25 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin Specific Peptidase 25 (USP25) antibodyUSP25 适用: 人 WB 宿主: 小鼠 Monoclonal 7C1 unconjugated
anti-Ubiquitin Specific Peptidase 25 (USP25) (AA 1-230) antibodyUSP25 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin Specific Peptidase 25 (USP25) (AA 1037-1068), (C-Term) antibodyUSP25 适用: 人, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB4365 unconjugated
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应用备注
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Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat, Monkey
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Meulmeester, E., Kunze, M., Hsaio, H. H., Urlaub, H., Melchior, F. Mechanism and consequences for paralog-specific sumoylation of ubiquitin-specific protease 25. Molec. Cell 30: 610-619, 2008. 2. Valero, R., Bayes, M., Sanchez-Font, M. F., Gonzalez-Angulo, O., Gonzalez-Duarte, R., Marfany, G. Characterization of alternatively spliced products and tissue-specific isoforms of USP28 and USP25. Genome Biol. 2: research0043, 2001. Note: Electronic Article. 3. Valero, R., Marfany, G., Gonzalez-Angulo, O., Gonzalez-Gonzalez, G., Puelles, L., Gonzales-Duarte, R. USP25, a novel gene encoding a deubiquitinating enzyme, is located in the gene-poor region 21q11.2. Genomics 62: 395-405, 1999. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- USP25 (Ubiquitin Specific Peptidase 25 (USP25))
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别名
- USP25
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背景
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Synonyms: Ubiquitin carboxyl-terminal hydrolase 25, Deubiquitinating enzyme 25, USP on chromosome 21, Ubiquitin thioesterase 25, Ubiquitin-specific-processing protease 25, USP25, USP21
Tissue Specificity: Isoform USP25a is found in most adult and fetal tissues, expression is moderately high in testis, pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, but very low in peripheral blood, colon, small intestine, ovary, prostate, thymus and spleen. Isoform USP25b is found in all tissues except heart and skeletal muscle. Isoform USP25m is heart and skeletal muscle specific.
Background: Ubiquitin specific peptidase 25 is a protein that in humans is encoded by the USP25 gene. It is mapped to 21q21.1. Ubiquitin is a highly conserved 76-amino acid protein involved in regulation of intracellular protein breakdown, cell cycle regulation, and stress response. Ubiquitin is released from degraded proteins by disassembly of the polyubiquitin chains, which is mediated by ubiquitin-specific proteases (USPs), such as USP25.
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分子量
- 125 kDa
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基因ID
- 29761
抗原
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