eGFP 抗体 (AA 1-239)
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Quick Overview for eGFP 抗体 (AA 1-239) (ABIN7596110)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 1-239
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原理
- EGFP antibody
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纯化方法
- protein-A affinity chromatography
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免疫原
- Recombinant EGFP(1-239aa) purified from E. coli
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亚型
- IgG2a kappa
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anti-Enhanced Green Fluorescent Protein (eGFP) (AA 1-238) antibody
eGFP 适用: 人, 大鼠 WB, ELISA, IF (p), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Enhanced Green Fluorescent Protein (eGFP) antibodyeGFP 适用: 标记 WB, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-Enhanced Green Fluorescent Protein (eGFP) antibodyeGFP 适用: 标记 WB 宿主: 小鼠 Monoclonal unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycero
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- eGFP (Enhanced Green Fluorescent Protein (eGFP))
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别名
- EGFP
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背景
- Green fluorescent protein (GFP) here refers to the protein first purified from jellyfish Aequorea victoria, though many other organisms have similar proteins. It is a 26.9 kDa protein (composed of 238 a.a. residues) that shows green fluorescence in shortwave light (blue to ultraviolet). Despite of wild-type GFP, many mutants of GFP have been engineered for wider usage in research. Enhanced GFP (eGFP) has S65T and F64L mutations, which make GFP show increased fluorescence and fold more efficiently under 37 degrees C, respectively. eGFP allows the use of GFP in mammalian cells. In A. Victoria, GFP plays roles as an energy transfer acceptor. It has long been used in cell and molecular biology as a reporter of gene expression. GFP can also been applied as a molecular thermometer to measure temperature accurately in fluids.
抗原
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