SARS-CoV-2 Spike 抗体 (AA 671-688)
Quick Overview for SARS-CoV-2 Spike 抗体 (AA 671-688) (ABIN7595334)
抗原
See all SARS-CoV-2 Spike 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 671-688
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原理
- Guinea pig antibody to SARS-Cov-2 spike polybasic region (671-688)
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特异性
- Specific for SARS-Cov-2 spike glycoprotein polybasic region
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交叉反应
- SARS Coronavirus-2 (SARS-CoV-2)
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纯化方法
- Whole serum
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免疫原
- Synthetic peptide "ASYQTQTNSPRRARSVA" aa 671-688 of SARS spike glycoprotein polybasic region conjugated to blue carrier protein was used as the antigen.
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anti-SARS-CoV-2 Spike (C-Term) antibody
适用: SARS Coronavirus-2 (SARS-CoV-2), SARS Coronavirus (SARS-CoV) ELISA, WB, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-SARS-CoV-2 Spike antibody适用: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Beta, SARS CoV-2 Gamma, SARS CoV-2 Alpha ELISA, WB 宿主: 兔 Polyclonal unconjugated
anti-SARS-CoV-2 Spike (AA 319-541) antibodyAnimal-Free 适用: SARS Coronavirus-2 (SARS-CoV-2) ELISA, WB, FACS, IF 宿主: 小鼠 Monoclonal AB68-A09 unconjugated Recombinant Antibody
anti-SARS-CoV-2 Spike antibody适用: SARS Coronavirus-2 (SARS-CoV-2) ELISA, WB 宿主: Human Monoclonal AM004414 (414-4) unconjugated Recombinant Antibody
anti-SARS-CoV-2 Spike antibody适用: SARS Coronavirus-2 (SARS-CoV-2) ELISA 宿主: Human Monoclonal AM015553 (553-15) unconjugated Recombinant Antibody
anti-SARS-CoV-2 Spike (AA 319-541) antibody适用: SARS Coronavirus-2 (SARS-CoV-2) ELISA, FACS 宿主: 兔 Monoclonal DM54 unconjugated Recombinant Antibody
anti-SARS-CoV-2 Spike (RBD) antibody适用: SARS Coronavirus-2 (SARS-CoV-2) WB 宿主: 山羊 Polyclonal unconjugated
anti-SARS-CoV-2 Spike antibodyResearch Grade 适用: SARS Coronavirus-2 (SARS-CoV-2) ELISA, FACS 宿主: Human Monoclonal CR3022 unconjugated Recombinant Antibody
anti-SARS-CoV-2 Spike (RBD) antibodyVerified 适用: SARS Coronavirus-2 (SARS-CoV-2) ELISA, WB 宿主: 山羊 Polyclonal unconjugated
anti-SARS-CoV-2 Spike (RBD) antibody适用: SARS Coronavirus-2 (SARS-CoV-2) ELISA, FACS 宿主: 兔 Monoclonal DM26 unconjugated
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应用备注
- IHC WB. A dilution of 1: 1000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
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储存条件
- 4 °C,-20 °C
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储存方法
- Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
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有效期
- 12 months
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- SARS-CoV-2 Spike
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背景
- Function: Spike protein S1:attaches the virion to the cell membrane by interacting with host receptor initiating the infection. Binding to human ACE2 receptor and internalization of the virus into the endosomes of the host cell induces conformational changes in the Spike glycoprotein. The stalk domain of S contains three hinges giving the head unexpected orientational freedom. Uses human TMPRSS2 for priming in human lung cells which is an essential step for viral entry. Can be alternatively processed by host furin. Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes. Spike protein S2: mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model the protein has at least three conformational states: pre-fusion native state pre-hairpin intermediate state and post-fusion hairpin state. During viral and target cell membrane fusion the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes. Spike protein S2': Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis. Spike glycoprotein: May down-regulate host tetherin (BST2) by lysosomal degradation thereby counteracting its antiviral activity.
抗原
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