GPR183 抗体 (Extracellular) (FITC)
Quick Overview for GPR183 抗体 (Extracellular) (FITC) (ABIN7582034)
抗原
See all GPR183 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 18-31, Extracellular
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原理
- A Rabbit Polyclonal Antibody to GPR183 (extracellular) conjugated to the fluorescent dye FITC
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序列
- (C)GND(S)DLYAHHSTAR
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特异性
- Extracellular, N-terminus.
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预测反应
- Human - 13 out of 14 amino acid residues identical Mouse,rat - 12 out of 14 amino acid residues identical
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产品特性
- Anti-GPR183 (extracellular) Antibody (ABIN7043152, ABIN7044457 and ABIN7044458) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunohistochemistry and live cell flow cytometry applications. It has been designed to recognize GPR183 from mouse, rat and human samples. Anti-GPR183 (extracellular)-FITC Antibody (ABIN7043152, ABIN7044457 and ABIN7044458-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging. It has been designed to recognize GPR183 from mouse, rat and human samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
- (C)GND(S)DLYAHHSTAR, corresponding to amino acid residues 18 - 31 of human GPR183
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亚型
- IgG
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anti-G Protein-Coupled Receptor 183 (GPR183) (AA 18-31), (Extracellular) antibody
GPR183 适用: 人 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 183 (GPR183) (AA 1-31) antibodyGPR183 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 183 (GPR183) (Internal Region) antibodyVerified GPR183 适用: 人 ELISA, FACS, IF 宿主: 山羊 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 183 (GPR183) (Internal Region) antibodyGPR183 适用: 人, 小鼠 ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 183 (GPR183) (Extracellular Domain) antibodyGPR183 适用: 人, 猴 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 183 (GPR183) (Cytoplasmic Domain) antibodyGPR183 适用: 人, 小鼠, 大鼠 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 183 (GPR183) (AA 1-361) antibodyGPR183 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 183 (GPR183) (AA 300-350) antibodyGPR183 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: N/A
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说明
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Negative Control: (ABIN7582044)
Blocking Peptide: (ABIN7235392)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 15 μL or 50 μL double distilled water (DDW), depending on the sample size.
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4, 1 % BSA with 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- GPR183 (G Protein-Coupled Receptor 183 (GPR183))
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别名
- GPR183
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背景
- G-Protein Coupled Receptor 183, Epstein-Barr Virus-Induced G-Protein Coupled Receptor 2, EBI2,G-protein coupled receptor 183 (GPR183) also known as Epstein-Barr virus-induced gene 2 (EBI2), is a chemotactic receptor that participates in the migratory capability of cells 2. It belongs to the rhodopsin-like subfamily of class A transmembrane spanning (7TM) G protein-coupled receptors and its most potent ligand is oxysterol 7α-25-OHC 1.Dihydroxycholesterols, such as oxysterol 7α-25-OHC, are generated from cholesterol in steady state but also in the context of inflammation. GPR183 senses these oxysterols and induces chemotactic migration of immune cells towards higher concentrations of these ligands and hence cells that express GPR183 are trafficking through an oxysterol gradient dependent manner acting like chemokine processes. The migratory function of this receptor affects several important immune processes, in particular, GPR183 is involved in the T-dependent antibody response in the germinal centers. This puts GPR183 in a unique position to effect autoimmunity and inflammatory bowel diseases 3,4.In addition, GPR183 is highly expressed by type 3 innate lymphoid cells (ILC3) and its oxysterol ligand was shown to be essential for the localization and the migration of ILC3s and to have a critical role for the formation of lymphoid tissues in the mouse colon during colitis 2.
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基因ID
- 1880
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UniProt
- P32249
抗原
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