GPR171 抗体 (Extracellular) (PE)
Quick Overview for GPR171 抗体 (Extracellular) (PE) (ABIN7582033)
抗原
See all GPR171 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 155-169, Extracellular
-
原理
- A Rabbit Polyclonal Antibody to GPR171 (extracellular) conjugated to the fluorescent dye Phycoerythrin (PE)
-
序列
- Peptide (C)KDIKEKSNVG (S)MEFK
-
特异性
- 2nd extracellular loop
-
预测反应
- Mouse,15 amino acid residues identical, rat - 13,human - 14
-
产品特性
- Anti-GPR171 (extracellular) Antibody (ABIN7043150, ABIN7044447 and ABIN7044448) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, indirect flow cytometry, and live cell imaging applications. It has been designed to recognize GPR171 from human, mouse and rat samples. Anti-GPR171 (extracellular)-PE Antibody (ABIN7043150, ABIN7044447 and ABIN7044448-PE) is directly conjugated to the Phycoerythrin (PE) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.
-
纯化方法
- Affinity purified on immobilized antigen.
-
免疫原
- Peptide (C)KDIKEKSNVG(S)MEFK, corresponding to amino acid residues 155 - 169 of mouse GPR171.
-
亚型
- IgG
-
-
anti-G Protein-Coupled Receptor 171 (GPR171) (Extracellular Domain) antibody
GPR171 适用: 人, Cow, 猴, 兔 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 171 (GPR171) (Extracellular Domain) antibodyGPR171 适用: 人, 猴 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 171 (GPR171) (Cytoplasmic Domain) antibodyGPR171 适用: 人, 大鼠, Cow, 犬 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 171 (GPR171) (AA 155-169), (Extracellular) antibodyGPR171 适用: 小鼠 WB, FACS, LCI 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 171 (GPR171) (AA 100-200) antibodyGPR171 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 171 (GPR171) (AA 141-190) antibodyGPR171 适用: 人, 小鼠 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 171 (GPR171) (Internal Region) antibodyGPR171 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: N/A
-
说明
-
Negative Control: (ABIN7582046)
Blocking Peptide: (ABIN7235380)
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- 50 μL double distilled water (DDW).
-
浓度
- 1 mg/mL
-
缓冲液
- PBS pH 7.4, 1 % BSA with 0.05 % sodium azide
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
-
Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
-
-
- GPR171 (G Protein-Coupled Receptor 171 (GPR171))
-
别名
- GPR171
-
背景
- G-Protein Coupled Receptor 171, Platelet Activating Receptor Homolog, G-protein coupled receptor H963,G protein-coupled receptor 171 (GPR171) is an orphan G-protein coupled receptor (GPCR) activated by BigLEN, one of the most abundant neuropeptides in the brain that is derived from ProSAAS1. GPR171 contains seven transmembrane domains, an extracellular N-terminus and an intracellular C-terminal tail. Its detailed structure and ligand binding domain structure was predicted by homology modeling based on its similarity to P2Y12 receptor2,3.BigLEN-GPR171 neuropeptide-receptor system is expressed in the brain, at the basolateral amygdala (BLA) and the periaqueductal gray brain area4-6.This system plays an important role in regulating feeding-related behaviors, food intake and body weight regulation. It is also involved in anxiety behavior and various psychiatric disorders4,5.Furthermore, BigLEN-GPR171 are expressed in GABAergic neurons within the periaqueductal gray, a key brain area involved in pain modulation and opioid functions6.GPR171 is a potential target for drug development in obesity and weight problems, anxiety, various psychiatric disorders and pain.
-
基因ID
- 229323
-
UniProt
- Q8BG55
抗原
-
