GPR142 抗体 (Extracellular) (FITC)
Quick Overview for GPR142 抗体 (Extracellular) (FITC) (ABIN7582032)
抗原
See all GPR142 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 291-304, Extracellular
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原理
- A Rabbit Polyclonal Antibody to GPR142 (extracellular) conjugated to the fluorescent dye FITC
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序列
- (C)APVHRDWRVH LALD
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特异性
- Extracellular, 3rd loop.
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预测反应
- Rat,human - identical
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产品特性
- Anti-GPR142 (extracellular) Antibody (ABIN7237652, ABIN7316613 and ABIN7316614) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize GPR142 from rat, mouse and human samples. Anti-GPR142 (extracellular)-FITC Antibody (ABIN7237652, ABIN7316613 and ABIN7316614-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
- (C)APVHRDWRVHLALD, corresponding to amino acid residues 291 - 304 of mouse GPR142
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亚型
- IgG
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anti-G Protein-Coupled Receptor 142 (GPR142) (Internal Region) antibody
GPR142 适用: 人 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 142 (GPR142) antibodyGPR142 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 142 (GPR142) (AA 1-50) antibodyGPR142 适用: 人 WB, ELISA, IF, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 142 (GPR142) (Internal Region) antibodyVerified GPR142 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 142 (GPR142) (AA 1-50) antibodyGPR142 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 142 (GPR142) (AA 86-114), (N-Term) antibodyGPR142 适用: 人 WB, FACS 宿主: 兔 Polyclonal RB28809 unconjugated
anti-G Protein-Coupled Receptor 142 (GPR142) (AA 93-122), (N-Term) antibodyGPR142 适用: 人 WB, EIA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: N/A
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说明
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Negative Control: (ABIN7582044)
Blocking Peptide: (ABIN7235372)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 50 μL double distilled water (DDW).
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4, 1 % BSA with 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- GPR142 (G Protein-Coupled Receptor 142 (GPR142))
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别名
- GPR142
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背景
- G-Protein Coupled Receptor 142, Probable G-Protein Coupled Receptor 142, PGR2,G-protein-coupled receptors (GPCRs) are the largest family of membrane proteins and mediate a myriad of physiological and pathophysiological signaling processes. Of these receptors, all are seven membrane spanning receptors but not all are G protein binding but it is convenient to refer to the receptors as GPCRs1.The GPR142 is a GPCR selectively activated by aromatic amino acids and share 33 % amino acid identity with GPR139. L-tryptophan representing one of the most potent ligands, having an EC50 values of 0.2 - 1 mM2. GPR142 is expressed in both rodent and human pancreatic beta cells. Moreover, it was found to be implicated in the regulation of insulin, thereby having a crucial role in Type II diabetes management3. Moreover, GPR142 is highly expressed in immune cells and its ligand L-tryptophan and metabolite serotonin are known to take part in inflammation process. It was shown that mice lacking GPR142 gene are more resilient to anti-type II collagen (CII) antibody-induced arthritis however the exact mechanism is currently unknown4.
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基因ID
- 217302
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UniProt
- Q7TQN9
抗原
-
