TMEM175 抗体 (Loop 4, Lumenal Region)
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Quick Overview for TMEM175 抗体 (Loop 4, Lumenal Region) (ABIN7581993)
抗原
适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 278-291, Loop 4, Lumenal Region
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原理
- A Rabbit Polyclonal antibody to TMEM175
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序列
- CEDNVPDPKD VQEK
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预测反应
- Rat - 13 out of 14 amino acid residues identical Human - 12 out of 14 amino acid residues identical
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产品特性
- Anti-TMEM175 Antibody (ABIN7581993) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize TMEM175 from rat, mouse and human samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
- CEDNVPDPKDVQEK, corresponding to amino acid residues 278 - 291 of mouse TMEM175
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亚型
- IgG
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anti-Transmembrane Protein 175 (TMEM175) (AA 34-83) antibody
TMEM175 适用: 小鼠, 大鼠, 人, 犬, Hamster, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Transmembrane Protein 175 (TMEM175) (AA 34-83) antibodyTMEM175 适用: 小鼠, 大鼠, 人, 犬, Hamster, 猴, Cow WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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WB: 1:200
FC: The optimal concentration should be determined by the user
ICC: The optimal concentration should be determined by the user
IHC: 1:300
IP: The optimal concentration should be determined by the user
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.
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浓度
- 0.8 mg/mL
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缓冲液
- PBS pH 7.4, 1 % BSA with 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
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- TMEM175 (Transmembrane Protein 175 (TMEM175))
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别名
- TMEM175
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背景
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Synonyms: Transmembrane Protein 175, Endosomal/Lysosomal Proton Channel TMEM175
Description: Transmembrane protein 175, TMEM175, is an endosomal/lysosomal proton channel that plays a crucial role in preserving the lysosomal membrane potential and maintaining pH stability. TMEM175 mediates lysosomal proton efflux, known as the "H+ leak" pathway, preventing excessive acidification of lysosomes.1 Intracellular endosomes and lysosomes rely on a vacuolar-type H+ ATPase (V-ATPase) to establish a significant proton concentration gradient across their membranes, which plays a crucial role in facilitating active transport of ions and metabolites between the vesicular lumen and the cytosol. To effectively digest macromolecules, lysosomes rely on an acidic environment with a pH range of 4.5 to 5.0, which is maintained through the entry of protons by the V-ATPase pump and their exit through the "H+ leak" pathway, which is the passive release of H+ into the cytosol. TMEM175 functions as a proton-activated, proton-selective channel on the lysosomal membrane in order to mediate the "H+ leak" pathway, prohibiting the excessive acidification of lysosomes. When lysosomes are acidified beyond the typical range, TMEM175 is activated, prohibiting further lysosomal acidification.1Mammalian TMEM175 consists of two homologous 6-TM domains and is suggested to function as a dimer. TM1 serves as the inner helix lining the pore and forms an hourglass-shaped ion permeation pathway within the channel. The C-terminal half of TM1 contains hydrophobic residues that create a bottleneck along the ion conduction pathway, serving as the selectivity filter of the channel. TMEM175 conducts K+ constitutively at pH 7.4, but shows reduced K+ permeation at lower pH . Conversely, proton current through TMEM175 increases with decreasing pH due to the increased proton gradient. Molecular dynamics simulation, structure-based mutagenesis, and electrophysiological analysis suggest that K+ ions and protons share the same permeation pathway in TMEM175.2,3Altered lysosomal pH disrupts various cellular processes, including lysosomal degradation, cargo loading, catabolite export, vesicle movement, and nutrient sensing. This impairment contributes to the pathogenesis of neurodegenerative diseases such as Parkinson's disease, Alzheimer's disease, and lysosomal storage diseases. Optimization of lysosomal pH can effectively clear toxic protein aggregates associated with these diseases. Thus, maintaining lysosomal pH homeostasis plays a crucial role in lysosome function, cellular health, and neuropathology.1
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基因ID
- 72392
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UniProt
- Q9CXY1
抗原
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