TMEM150C 抗体 (Extracellular)
TMEM150C
适用: 大鼠
WB, IF (cc), IHC, LCI
宿主: 兔
Polyclonal
unconjugated
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- 抗原 See all TMEM150C products
- TMEM150C (Transmembrane Protein 150C (TMEM150C))
- 抗原表位
- AA 33-50, Extracellular
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适用
- 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This TMEM150C antibody is un-conjugated
- 应用范围
- Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (IHC), Live Cell Imaging (LCI)
- 原理
- A Rabbit Polyclonal antibody to TMEM150C/Tentonin 3 (extracellular).
- 序列
- (C)EDDKILPLNS AARKSGVK
- 特异性
- Extracellular, 1st loop.
- 预测反应
- Mouse - 17 out of 18 amino acid residues identical Human - 16 out of 18 amino acid residues identical
- 产品特性
- Anti-TMEM150C/Tentonin 3 (extracellular) Antibody (ABIN7581992) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize TMEM150C from mouse, rat and human samples.
- 纯化方法
- Affinity purified on immobilized antigen.
- 免疫原
- (C)EDDKILPLNSAARKSGVK, corresponding to amino acid residues 33 - 50 of rat TMEM150C
- 亚型
- IgG
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- 应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300
Application Dilutions Western blot wb: 1:200
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- 0.2 mL double distilled water (DDW).
- 浓度
- 1 mg/mL
- 缓冲液
- PBS pH 7.4
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- 抗原
- TMEM150C (Transmembrane Protein 150C (TMEM150C))
- 别名
- TMEM150C (TMEM150C 产品)
- 背景
- Transmembrane Protein 150C, TTN3,Transmembrane protein 150C (TMEM150C) also known as Tentonin-3 (TTN3), is a small protein composed of 249 amino acids comprising six transmembrane domains.TMEM150C which was first thought to be a mechanosensitive ion channel that confers a relatively slow inactivating current in proprioceptive neurons in mouse dorsal root ganglion (DRG)1. Later studies, however, showed that the mechanosensitive currents attributed to TMEM150C in different cell setups, could be attributed to the presence of endogenous Piezo1 channels2.Although, some controversy still remains, it is generally accepted that TMEM150C does not form a mechanosensitive channel per se, but rather it functions as a general regulator or sensor, of mechanosensitive channels like Piezo1, Piezo2 and TREK1 (K2P2.1) 2,3.TMEM150C has also been described in the aortic arch and nodose ganglia neurons, where it is involved in the mechanotransduction of arterial pressure to electrical signals in baroreceptors. Dysregulation of TMEM150C alters the sensitivity of the baroreflex, a cardiovascular reflex that is essential for blood pressure homeostasis, leading to diseases such as hypertension, stroke, and heart failure 4.Furthermore, recent evidence suggests that TMEM150C functions as a stretch-activated cation channel in pancreatic β cells, contributing to insulin secretion induced by high glucose levels and hypotonicity. Whether TMEM150C functions in this context as a stretch activated channel itself or whether it functions in conjunction with other proposed stretch-activated channels like TRPV4 or TRPV2, remains to be seen 3,5.
- 基因ID
- 360916
- UniProt
- B5DFH9
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