PKD2 抗体 (C-Term, Intracellular)
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Quick Overview for PKD2 抗体 (C-Term, Intracellular) (ABIN7886148)
抗原
See all PKD2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 914-927, C-Term, Intracellular
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原理
- A Rabbit Polyclonal Antibody to TRPP1 (PKD2) Channel
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序列
- (C)ERWESDDAAS QISH
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预测反应
- 14 amino acid residues identical, rat - 11,Mouse - 12
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产品特性
- Anti-TRPP1 (PKD2) Antibody (ABIN7581944) is a highly specific antibody directed against an epitope of human Polycystin-2. The antibody can be used in western blot, immunohistochemistry and immunocytochemistry applications. It has been designed to recognize TRPP1 from human, rat, and mouse samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
- (C)ERWESDDAASQISH, corresponding to amino acid residues 914-927 of human TRPP1
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亚型
- IgG
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anti-Polycystic Kidney Disease 2 (Autosomal Dominant) (PKD2) (AA 261-360) antibody
PKD2 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 4C8 unconjugated
anti-Polycystic Kidney Disease 2 (Autosomal Dominant) (PKD2) (AA 621-832) antibodyPKD2 适用: 人 WB, ELISA, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Polycystic Kidney Disease 2 (Autosomal Dominant) (PKD2) (AA 327-539) antibodyPKD2 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Polycystic Kidney Disease 2 (Autosomal Dominant) (PKD2) (AA 261-360) antibodyPKD2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1G3 unconjugated
anti-Polycystic Kidney Disease 2 (Autosomal Dominant) (PKD2) (AA 680-968) antibodyPKD2 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Polycystic Kidney Disease 2 (Autosomal Dominant) (PKD2) (AA 240-410) antibodyPKD2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Polycystic Kidney Disease 2 (Autosomal Dominant) (PKD2) (C-Term) antibodyPKD2 适用: 人 WB, IP, IHC (fp) 宿主: 兔 Polyclonal unconjugated
anti-Polycystic Kidney Disease 2 (Autosomal Dominant) (PKD2) (pSer876) antibodyPKD2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Polycystic Kidney Disease 2 (Autosomal Dominant) (PKD2) (AA 918-968) antibodyPKD2 适用: 人, 小鼠 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Polycystic Kidney Disease 2 (Autosomal Dominant) (PKD2) antibodyPKD2 适用: 人 WB, FACS 宿主: 小鼠 Monoclonal 25GB655 unconjugated
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应用备注
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WB: 1:200
FC: The optimal concentration should be determined by the user
ICC: 1:50-1:100
IHC: 1:100
IP: The optimal concentration should be determined by the user
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.
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浓度
- 0.8 mg/mL
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缓冲液
- PBS pH 7.4, 1 % BSA with 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
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- PKD2 (Polycystic Kidney Disease 2 (Autosomal Dominant) (PKD2))
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别名
- PKD2
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背景
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Synonyms: Polycystin-2, Polycystic kidney disease 2 protein, PC2
Description: Transient receptor potential (TRP) channels are relatively non-selective ion channels that enable the flow of cations down their electrochemical gradient. This enables the increase in intracellular Na+ and Ca2+ concentrations and ultimately in the cell membrane depolarization, which is important for action potential propagation and muscle contraction1. They are activated by a broad range of stimuli, namely temperature, voltage, pH , endocrine factors, as well as signaling molecules2.The TRP channel family is composed of 28 members divided into 7 subgroups: TRPV, TRPC, TRPM, TRPA, TRPN, TRPP and TRPML. All members of the TRP family have 6 transmembrane (TM) domains, with the pore between the fifth (S5) and sixth (S6) TM domains. In general, TRP channels enable the passage of either Na+ or Ca2+ ions with little to no preference.However, some channels do exhibit some selectivity. Also, TRP channels do not display the positive charges in the S4 voltage-sensing domain like most voltage-sensitive channels, although they do display voltage-dependency3. In addition, the C-terminal, located intracellularly, contains a TRP domain comprising 25 amino acids that are highly conserved between TRP channels. Within the TRP domain, there is a TRP box composed of six amino acids, and TRP box 2 - a proline rich domain1,3. The TRP domain seems to be responsible for the binding of PIP2, a phospholipid important for the regulation of channel activity4.TRPP1 (polycystin-2, PC2, PKD2) belongs to the TRPP subfamily along with TRPP3 and TRPP5 proteins, and forms non-selective cation channels with different permeability to various divalent cations5,6. The cellular localization of TRPP1 has been and still is the subject of a lasting debate. In many cell-types, TRPP1 is retained in the endoplasmic reticulum (ER) where it most likely functions as a Ca2+ release channel7,8, and with the help of cofactors, TRPP2 reaches the plasma membrane and the cilia8. TRPP1 expression is widespread and is best characterized for its expression in the kidney where it is developmentally regulated9,10. In the kidney, it associates with PKD1 (TRPP2) an eleven TM-spanning protein (which does not belong to the TRP superfamily) to form functional channels11. In addition, TRPP1 is identified as one of the genes responsible for autosomal dominant polycystic kidney disease (ADPKD)5,12.
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基因ID
- 5311
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UniProt
- Q13563
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途径
- cAMP Metabolic Process, Maintenance of Protein Location, Negative Regulation of Transporter Activity
抗原
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