PANX1 抗体 (Intracellular)
Quick Overview for PANX1 抗体 (Intracellular) (ABIN7581943)
抗原
See all PANX1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 18-31, Intracellular
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原理
- A Guinea Pig Polyclonal Antibody to Pannexin 1
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序列
- (C)KEPTEPKFKG LRLE
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特异性
- Intracellular, N-terminus
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预测反应
- Rat,mouse - identical
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产品特性
- Guinea Pig Anti-Pannexin 1 Antibody (ABIN7581943) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Pannexin 1 from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-Pannexin 1 Antibody (ABIN7043426, ABIN7044100 and ABIN7044101) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation and more.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
- (C)KEPTEPKFKGLRLE, corresponding to amino acid residues 18- 31 of human PANX1
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亚型
- IgG
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anti-Pannexin 1 (PANX1) (AA 77-260) antibody
PANX1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Pannexin 1 (PANX1) (Middle Region) antibodyPANX1 适用: 人, 大鼠, 小鼠, Cow, 犬, 豚鼠, 马, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Pannexin 1 (PANX1) (AA 228-411) antibodyPANX1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Pannexin 1 (PANX1) (AA 288-426) antibodyPANX1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Pannexin 1 (PANX1) (AA 327-425) antibodyPANX1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2E3 unconjugated
anti-Pannexin 1 (PANX1) (AA 231-280) antibodyPANX1 适用: 人, Cow, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Pannexin 1 (PANX1) (AA 297-426) antibodyPANX1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Pannexin 1 (PANX1) antibodyPANX1 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
anti-Pannexin 1 (PANX1) (Middle Region) antibodyPANX1 适用: 哺乳动物 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Pannexin 1 (PANX1) (Internal Region) antibodyPANX1 适用: 人, 大鼠, 小鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300
Application Dilutions Western blot wb: 1:400
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说明
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Negative Control: (ABIN7236221)
Blocking Peptide: (ABIN7236221)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 0.2 mL double distilled water (DDW).
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- PANX1 (Pannexin 1 (PANX1))
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别名
- PANX1
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背景
- PANX1, PX1, MRS1, UNQ2529,Gap junctions are usually found in clusters and enable intercellular communication by allowing the passage of small molecules between cells1. They play important roles in different biological processes. These include differentiation, cell cycle synchronization, cellular development, neuronal activity and the immune response2-4.Proteins involved in gap junction formation are composed of four transmembrane domains, two extracellular loops and one intracellular loop and intracellular N- and C-termini5,6. Several consensus cysteine residues are in the extracellular loop and are essential and necessary for intercellular docking of gap junction hemichannels in the opposing cell membrane5-7.Pannexins (Pannexin 1, Pannexin 2 and Pannexin 3) belong to the superfamily of gap junction proteins. Pannexin 1 (PANX1) is ubiquitously expressed, Pannexin 2 (PANX2) is specifically expressed in the human brain and widespread in rodents and Pannexin 3 (PANX3) is also detected in the brain8.The gating properties of Pannexins were studied in Xenopus oocytes and results demonstrate that only PANX1 is able to form homomeric hemichannels, and is also able to form heteromeric hemichannels with PANX2 but not with PANX39,10. Not surprising, PANX1 gating properties depend whether it forms homomeric or heteromeric hemichannels.A number of different stimuli are known to open these channels and include mechanical stress, extracellular ATP, increases in intracellular Ca2+ and inflammation9.Possible roles for Pannexins include paracrine signaling in vascular endothelial cells and taste cell signaling9. Pannexin-1 was shown to interact with NMDA receptors in epilepsy, ischemia, pain and other pathologies10.
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基因ID
- 25107
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UniProt
- Q96RD7
抗原
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