NPSR1 抗体 (Extracellular)
Quick Overview for NPSR1 抗体 (Extracellular) (ABIN7581923)
抗原
See all NPSR1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 297-312, Extracellular
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原理
- A Rabbit Polyclonal antibody to Neuropeptide S Receptor (extracellular)
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序列
- (C)DNFNVLPDTK ERFYAS
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特异性
- Extracellular, 3rd loop.
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预测反应
- Rat - 15 out of 16 amino acid residues identical,Human - 14 out of 16 amino acid residues identical
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产品特性
- Anti-Neuropeptide S Receptor (extracellular) Antibody (ABIN7581923) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize NPSR1 from mouse, rat and human samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
- (C)DNFNVLPDTKERFYAS, corresponding to amino acid residues 297 - 312 of mouse NPSR1
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亚型
- IgG
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anti-Neuropeptide S Receptor 1 (NPSR1) (AA 1-52) antibody
NPSR1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Neuropeptide S Receptor 1 (NPSR1) (Extracellular Domain) antibodyNPSR1 适用: 人, 小鼠, 大鼠, Hamster, 猴 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Neuropeptide S Receptor 1 (NPSR1) (AA 241-300) antibodyNPSR1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Neuropeptide S Receptor 1 (NPSR1) (AA 2-53) antibodyNPSR1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2F5 unconjugated
anti-Neuropeptide S Receptor 1 (NPSR1) (AA 2-53) antibodyNPSR1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Neuropeptide S Receptor 1 (NPSR1) (Internal Region) antibodyNPSR1 适用: 人, 小鼠, 大鼠 WB, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Neuropeptide S Receptor 1 (NPSR1) (C-Term) antibodyNPSR1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Neuropeptide S Receptor 1 (NPSR1) (2nd Extracellular Domain) antibodyNPSR1 适用: 人 IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:400
Application Dilutions Western blot wb: 1:200
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 0.2 mL double distilled water (DDW).
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- NPSR1 (Neuropeptide S Receptor 1 (NPSR1))
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别名
- NPSR1
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背景
- NPSR1, G-Protein Coupled Receptor 154, GPR154, G-Protein Coupled Receptor for Asthma Susceptibility, GPRA,The Neuropeptide S Receptor (NPSR1), also known as GPR154, GPRA, or VRR1, belongs to the G protein-coupled receptor (GPCR) superfamily and is activated by neuropeptide S (NPS)1,2.NPS is a 20-residue peptide, named due to the presence of a serine (S) residue in the N-terminal position of the molecule. NPS expression is limited to a few discrete brain areas, such as in isolated cells of the amygdala, the hypothalamus, and specific regions of the brainstem. The primary sequence of NPS is highly conserved among vertebrates especially at the N-terminus, which is crucial for biological activity. The positions 2, 3, 4, and 5 on N-terminus of NPS play important roles in bioactivity and activation of NPSR11,2.The Neuropeptide S Receptor is widely distributed in the brain particularly in regions that are associated with regulation of stress response, memory, the olfactory system, and regulation of arousal. NPSR1 can signal via both Gq and Gs to increase cellular excitability and inhibit cell growth. Via the Gq pathway, NPSR1 leads to activation of Gq-phospholipase C and cleavage of phospholipid phosphatidylinositol 4,5-bisphosphate to inositol 1,4,5-trisphosphate (IP3) and diacylglycerol. IP3 activates IP3 receptor and releases Ca2 + from the ER. In some neurons, store-operated calcium channels are also activated, allowing entry of extracellular calcium into the cells 2.In the Gs pathway, NPSR signaling activates adenylate cyclase, increased synthesis of cAMP, and activation of protein kinase A 2.NPSR1 activation, like other GPCRs, also leads to activation of extracellular signal-regulated kinase 1/2 (ERK1/2) 2. Knockout studies in mice demonstrated that the NPS system produces anti-aggressive effects, reduces social anxiety behavior, modulates fear response and enhances motor performance skills3,4. A growing body of evidence suggests a close relationship between neuropeptide S and its receptor NPSR1 system in Parkinson's disease (PD). Indeed, administration of NPS to the central nervous syatem, ameliorates motor and non-motor dysfunctions in PD models, suggesting that targeting the NPS/NPSR1 system might be beneficial for Parkinson-related signs and symptoms 5.
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基因ID
- 319239
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UniProt
- Q8BZP8
抗原
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