NPFFR2 抗体 (Extracellular)
Quick Overview for NPFFR2 抗体 (Extracellular) (ABIN7581922)
抗原
See all NPFFR2 (NPFF2) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 186-199, Extracellular
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原理
- A Rabbit Polyclonal antibody to Neuropeptide FF Receptor 2 (extracellular)
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序列
- (C)EEKYYRVRLS SHNK
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特异性
- Extracellular, 2nd loop.
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预测反应
- Rat - identical Human - 12 out of 14 amino acid residues identical
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产品特性
- Anti-Neuropeptide FF Receptor 2 (extracellular) Antibody (ABIN7581922) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot and indirect flow cytometry applications. It has been designed to recognize NPFFR2 from mouse, rat and human samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
- (C)EEKYYRVRLSSHNK, corresponding to amino acid residues 186 - 199 of mouse NPFFR2
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亚型
- IgG
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anti-Neuropeptide FF Receptor 2 (NPFF2) (Extracellular Domain) antibody
NPFF2 适用: 人, 马 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Neuropeptide FF Receptor 2 (NPFF2) (AA 251-300) antibodyNPFF2 适用: 人, 马, Cow WB 宿主: 兔 Polyclonal unconjugated
anti-Neuropeptide FF Receptor 2 (NPFF2) (AA 23-43) antibodyNPFF2 适用: 人 ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Neuropeptide FF Receptor 2 (NPFF2) (AA 23-43) antibodyNPFF2 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Neuropeptide FF Receptor 2 (NPFF2) (AA 1-420) antibodyNPFF2 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Neuropeptide FF Receptor 2 (NPFF2) (C-Term) antibodyNPFF2 适用: 人, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Neuropeptide FF Receptor 2 (NPFF2) (AA 1-140) antibodyNPFF2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Neuropeptide FF Receptor 2 (NPFF2) (C-Term) antibodyNPFF2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Neuropeptide FF Receptor 2 (NPFF2) (Middle Region) antibodyNPFF2 适用: 人, 马, Cow WB 宿主: 兔 Polyclonal unconjugated
anti-Neuropeptide FF Receptor 2 (NPFF2) (C-Term) antibodyNPFF2 适用: 人 IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: 1:200
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 0.2 mL double distilled water (DDW).
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- NPFFR2 (NPFF2) (Neuropeptide FF Receptor 2 (NPFF2))
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别名
- NPFFR2
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背景
- NPFFR2, G-Protein Coupled Receptor 74, GPR74,Neuropeptide FF receptor 2, NPFFR1, and also known as GPR74, is a member of the G protein-coupled receptor superfamily containing two subtypes, NPFFR1 and NPFFR2.1-2NPFFR1 and NPFFR2 are the high affinity receptors the endogenous neuropeptides NPFF and NPAF, also known as RFamide peptides, which are characterized by their Arg-Phe-NH2 motif at the C-terminal end.1-2NPFFR2 is expressed in both the central and peripheral nervous system, as well as in other organs such as heart, kidney and adipose tissue macrophages.1-4NPFFR2 has been extensively studied for its role in pain modulation. Mice over-expressing NPFFR2 showed more sensitivity to mechanical and thermal noxious stimuli. In addition, a role in the modulation of the development of opioid tolerance and the regulation of withdrawal symptoms, has been suggested.3,5NPFFR2 signaling has been implicated in the regulation of energy homeostasis, through the inhibition of food intake and the modulation of metabolic rate and energy expenditure.2,3,6 In this regard, it is interesting to note that, NPFFR2 has been identified in adipose tissue macrophages, cells that are involved in the development of obesity-induced metabolic diseases.7
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基因ID
- 104443
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UniProt
- Q924H0
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途径
- cAMP Metabolic Process
抗原
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