MEGF10 抗体 (Extracellular)
Quick Overview for MEGF10 抗体 (Extracellular) (ABIN7581914)
抗原
See all MEGF10 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 178-190, Extracellular
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原理
- A Rabbit Polyclonal antibody to MEGF10 (extracellular)
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序列
- CEDR(S)EQGTYGND
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特异性
- Extracellular, N-terminus.
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交叉反应 （详细）
- This antibody is specific for MEGF10, it won't recognize the closely related protein MEGF11 (Accession Q80T91)
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预测反应
- Mouse,rat,human - 12 out of 13 amino acid residues identical
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产品特性
- Anti-MEGF10 (extracellular) Antibody (ABIN7581914) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize MEGF10 from mouse, rat and human samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
- CEDR(S)EQGTYGND, corresponding to amino acid residues 178 - 190 of mouse MEGF10
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亚型
- IgG
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anti-Multiple EGF-Like-Domains 10 (MEGF10) (AA 55-150) antibody
MEGF10 适用: 小鼠 ELISA, IF (cc), IF (p), IHC (fro), IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Multiple EGF-Like-Domains 10 (MEGF10) (AA 26-160) antibodyMEGF10 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300
Application Dilutions Western blot wb: 1:400
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 0.2 mL double distilled water (DDW).
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浓度
- 0.8 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- MEGF10 (Multiple EGF-Like-Domains 10 (MEGF10))
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别名
- MEGF10
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背景
- Multiple EGF Like Domains 10, Multiple EGF-Like Domains Protein 10,MEGF10 is a member of the multiple epidermal growth factor-like domains protein family. MEGF10 is a type I transmembrane protein that consists of 15 EGF-like domains in its extracellular region and is the mammalian homolog of Draper (a Drosophila phagocytosis apoptotic cell receptor)1.MEGF10 is a mediator for apoptotic cell phagocytosis by central nervous system (CNS) macrophages- microglia and astrocytes, through binding with high affinity to C1q, an eat-me signal, that binds phosphatidylserine expressed on the surface of apoptotic cells2. Also necessary for astrocyte-dependent apoptotic neuron clearance in the developing cerebellum2. In this regard, MEGF10 works alongside other phagocytic receptors expressed in astrocytes or microglia such as the tyrosine kinase receptors MERTK and AXL, the G-protein coupled receptors Bai1 and GPR56 or the TREM2 receptor3.MEGF10 is involved in the uptake of amyloid-β peptide (Aβ42) in the brain. Alzheimer's disease (AD) is characterized by extracellular accumulation of senile plaques, the major component of which is the amyloid-β peptide (Aβ), and the presence of neurofibrillary tangles. MEGF10 as a functional receptor that mediates the uptake of amyloid-β peptide will help elucidate the molecular mechanisms of amlyoid-β clearance in Alzheimer's disease1,4.MEGF10 is also an essential factor in the regulation of myogenesis. It controls the balance between skeletal muscle satellite cells proliferation and differentiation through regulation of the notch signaling pathway5. In fact, mutations in the MEGF10 gene are the underlying cause of Early-onset myopathy, areflexia, respiratory distress, and dysphagia (EMARDD), a lethal congenital myopathy characterized by hypotonia with respiratory distress 5,6.
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基因ID
- 70417
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UniProt
- Q6DIB5
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途径
- Regulation of Muscle Cell Differentiation
抗原
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