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Leptin Receptor 抗体 (Extracellular)

Name: Leptin Receptor 抗体 (Extracellular)
SKU: ABIN7581913
Availability: OutOfStock

This anti-Leptin Receptor antibody is a 兔多克隆 antibody detecting Leptin Receptor in WB, IHC, FACS 和 LCI. Suitable for 小鼠.

产品编号 ABIN7581913

发货至: 中国

Quick Overview for Leptin Receptor 抗体 (Extracellular) (ABIN7581913)

抗原

See all Leptin Receptor (LEPR) 抗体

Leptin Receptor (LEPR)

适用

小鼠

宿主

兔

克隆类型

多克隆

标记

This Leptin Receptor antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Discover our top product LEPR Primary Antibody

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AA 777-791, Extracellular

原理

A Rabbit Polyclonal antibody to Leptin Receptor (extracellular)

序列

(C)EDDGMKWLRI PSNVK

特异性

Extracellular, N-terminus.

预测反应

Rat - 13 out of 15 amino acid residues identical Won't recognize Leptin Receptor from human samples

产品特性

Anti-Leptin Receptor (extracellular) Antibody (ABIN7581913) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize Leptin Receptor from mouse and rat samples. The antibody will not work with human samples.

纯化方法

Affinity purified on immobilized antigen.

免疫原

(C)EDDGMKWLRIPSNVK, corresponding to amino acid residues 777 - 791 of mouse LEPR

亚型

IgG
anti-Leptin Receptor (LEPR) (AA 901-1000) antibody
LEPR 适用: 人, 小鼠, 大鼠, Pig WB, ELISA, FACS, IF (p), IHC (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Leptin Receptor (LEPR) (AA 310-539) antibody
LEPR 适用: 人 WB, ELISA, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Leptin Receptor (LEPR) (AA 22-203) antibody
LEPR 适用: 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Leptin Receptor (LEPR) (AA 238-375) antibody
LEPR 适用: 大鼠 WB, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated

anti-Leptin Receptor (LEPR) (Internal Region) antibody
Verified LEPR 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, FACS 宿主: 山羊 Polyclonal unconjugated

anti-Leptin Receptor (LEPR) antibody
LEPR 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated

anti-Leptin Receptor (LEPR) (AA 23-52) antibody
LEPR 适用: 人 WB, ELISA, IHC, FACS 宿主: 兔 Polyclonal unconjugated

anti-Leptin Receptor (LEPR) (AA 501-600) antibody
LEPR 适用: 人, 小鼠, 大鼠 WB, FACS, IF (p), IHC (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Leptin Receptor (LEPR) (C-Term) antibody
LEPR 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated

anti-Leptin Receptor (LEPR) (AA 31-130) antibody
LEPR 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2H5 unconjugated

应用备注

Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300
Application Dilutions Western blot wb: 1:200

限制

仅限研究用
状态

Lyophilized

溶解方式

0.2 mL double distilled water (DDW).

浓度

1 mg/mL

缓冲液

PBS pH 7.4

储存条件

4 °C,-20 °C

储存方法

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
抗原

Leptin Receptor (LEPR)

别名

LEPR

背景

LEPR, OB receptor, OBR, CD295,Leptin receptor (LEP-R), also known as OB receptor (OB-R), HuB219, and CD295 is a receptor for the hormone leptin, which is involved in energy homeostasis and hunger/satiation cues.1LEP-R, encoded by the LEPR gene, is composed of 1165 amino acids with the extracellular domain consisting an N-terminal cytokine receptor homology domain (CRH-1), an immunoglobulin-like (Ig) domain, a second CRH domain (CRH-2) which is also known as the leptin-binding domain (LBD) and two Fibronectin type III (FNIII) domains. LEP-R forms a high-affinity 1:1 ratio complex with leptin in solution, but does not form the 2:1 ratio complex associated with the small cytokine receptors, such as growth hormone receptor (GHR). The 1:1 ratio interaction occurs at leptin's binding site II and can be blocked by mutations in this region. Mutations within the Ig domain of LEP-R and binding site III of leptin have been shown to inhibit transduction without disrupting receptor binding, indicating that the leptin-signaling complex forms a crossover arrangement between two leptin-LEP-R complexes.1Leptin is produced in adipose tissue in proportion to triglyceride stores and serves as an indicator of long-term energy status. Leptin acts mainly in the brain, specifically the hypothalamus, where its action is integrated with that of other adipokines, gastrokines, and other signals to coordinate energy homeostasis. Once secreted into the circulation, leptin travels to the brain where it enters the CNS likely via the choroid plexus and circumventricular organs. In the brain, leptin acts by binding and activating LEP-R, which is primarily expressed on specialized subsets of neurons in certain hypothalamic and brainstem nuclei.2Lack of leptin, such as in cases of starvation or genetic leptin deficiency, increases hunger while promoting an energy-sparing cascade of neuroendocrine and autonomic changes, including decreased sympathetic nervous system tone, thyroid function, growth, reproduction, and a variety of behavioral and physiological changes to respond appropriately to low stores of energy.2

基因ID

16847

UniProt

P48356

途径

JAK/STAT Signaling, AMPK Signaling, Feeding Behaviour