GPR143 抗体 (Intracellular)
Quick Overview for GPR143 抗体 (Intracellular) (ABIN7581885)
抗原
See all GPR143 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 225-239, Intracellular
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原理
- A Rabbit Polyclonal antibody to GPR143
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序列
- (C)KGRKGVYTEN ERLMG
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特异性
- Intracellular, 3rd loop.
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预测反应
- Rat- identical, human - 12 out of 15 amino acid residues identical
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产品特性
- Anti-GPR143 Antibody (ABIN7581885) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GPR143 from rat, mouse and human samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
- (C)KGRKGVYTENERLMG, corresponding to amino acid residues 225 - 239 of mouse GPR143
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亚型
- IgG
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anti-G Protein-Coupled Receptor 143 (GPR143) (AA 1-424) antibody
GPR143 适用: 人 WB, FACS 宿主: 小鼠 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 143 (GPR143) (AA 314-404) antibodyGPR143 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300
Application Dilutions Western blot wb: 1:400-1:500
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 0.2 mL double distilled water (DDW).
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- GPR143 (G Protein-Coupled Receptor 143 (GPR143))
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别名
- GPR143
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背景
- G-Protein Coupled Receptor 143, Ocular Albinism Type 1 Protein, OA1,G-protein coupled receptor 143, GPR143, also known as ocular albinism type 1 protein, OA1, is an orphan GPCR expressed intracellularly in pigment-producing cells on the melanosomal membrane and serves to regulate melanosome size and mediate protein transport to the melanosome via the endolysosomal system.1GPR143 is encoded by the OA1 gene, composed of 404 amino acids with 7 transmembrane helices, and associates with several Gα and Gβ subunits, as well β-Arrestin. Potential ligands for GPR143 include L-3,4-dihydroxyphenylalanine (L-DOPA) and dopamine. High dose L-DOPA treatment leads to intracellular Ca2+ influx and β-Arrestin recruitment. GPR143 has two sorting signals, a dileucine motif in ICL3 and a tryptophan-glutamic acid doublet in the C-terminal tail, which are necessary for lysosomal and melanosomal localization. Functional components of endosomal sorting complexes required for transport (ESCRT) are essential for intracellular sorting and ubiquitination. Due to the unique localization of GPR143 within the cell and its topological orientation, ligands are likely required to bind from the organelle lumen.1GPR143 is largely expressed on the melanosomal membrane of melanocytes, the pigment producing cells that are responsible for producing melanin in the skin and eyes. Melanin is a pigment that is crucial for the protection of skin and eyes from damaging UV light, as well as for the normal development of the optic system and eye function. Mutations in the OA1 gene and its subsequent protein products lead to ocular albinism due to melanosome malformation. Albinism is a pigment disorder characterized by decreased melanin in affected tissues, which leads to adverse effects including foveal hypoplasia and chiasmal misrouting.1,3GPR143 pathophysiology is also implicated in various degenerative diseases, including macular degeneration and Parkinson's disease, as well as playing a possible role in cancer susceptibility and progression.1,2
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基因ID
- 18241
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UniProt
- P70259
抗原
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