G Protein-Coupled Receptor 12 抗体 (Extracellular)
Quick Overview for G Protein-Coupled Receptor 12 抗体 (Extracellular) (ABIN7581884)
抗原
See all G Protein-Coupled Receptor 12 (GPR12) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 3-15, Extracellular
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原理
- A Rabbit Polyclonal antibody to SORCS2
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序列
- (C)EDPKVNLSGL PRD
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特异性
- Extracellular, N-term.
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预测反应
- Rat - identical,Human - 12 out of 13 amino acid residues identical
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产品特性
- Anti-GPR12 (extracellular) Antibody (ABIN7581884) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GPR12 from rat, mouse and human samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
- (C)EDPKVNLSGLPRD, corresponding to amino acid residues 3 - 15 of mouse GPR12
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亚型
- IgG
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anti-G Protein-Coupled Receptor 12 (GPR12) (AA 1-48) antibody
GPR12 适用: 人 ELISA, WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 12 (GPR12) (Extracellular Domain) antibodyGPR12 适用: 人, 小鼠, 大鼠, 马, Pig, Cow, 兔, Hamster IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 12 (GPR12) (Extracellular Domain) antibodyGPR12 适用: 人, 小鼠, 大鼠, 马, Pig, Cow, 犬, 猴, Hamster IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 12 (GPR12) antibodyGPR12 适用: 人, 小鼠, 大鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 12 (GPR12) (N-Term) antibodyGPR12 适用: 人 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 12 (GPR12) (C-Term) antibodyGPR12 适用: 人, 小鼠, 大鼠 ELISA, WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300
Application Dilutions Western blot wb: 1:200
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 0.2 mL double distilled water (DDW).
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- G Protein-Coupled Receptor 12 (GPR12)
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别名
- GPR12
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背景
- G-Protein Coupled Receptor 12, GPCR21,G-protein-coupled receptors (GPCRs), are the largest family of membrane proteins and mediate most of the physiological responses to hormones, neurotransmitters and environmental stimulants. At the most basic level, all GPCRs are characterized by the presence of seven membrane-spanning α-helical segments separated by alternating intracellular and extracellular loop regions1. Orphan GPCRs is a group of GPCRs that their endogenous ligands remain unidentified and therefore their natural functions in doubt2.GPR12 is an orphan G protein-coupled receptor related phylogenetically, functionally, and pharmacologically with the cannabinoid system. It considered as a "constitutively active receptor" since it greatly stimulates adenylate cyclase in the absence of ligand-receptor interaction. GPR12 seems to couple Gαs as well as Gαi proteins since it stimulates baseline cAMP-dependent signaling by activating Gαs proteins, and also inhibits forskolin activated signaling through Gαi interaction3.GPR12 expressed in the pituitary gland, in neurons in the frontal cortex, piriform cortex, thalamus, hypothalamus, hippocampus, amygdala, and olfactory bulb. It was found that GPR12 plays a significant role in energy balance. GPR12 knock out mice exhibit increase body weight and body fat mass. In addition, overexpression of GPR12 leads to cancer cells migration and invasion via phosphorylation of Keratin 84.Furthermore, GPR12 has been identified as an inverse agonist of the phytocannabinoid cannabidiol (CBD)4.
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基因ID
- 14738
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UniProt
- P35412
抗原
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