Gephyrin 抗体 (Intracellular)
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Quick Overview for Gephyrin 抗体 (Intracellular) (ABIN7581882)
抗原
See all Gephyrin (GPHN) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 183-195, Intracellular
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原理
- A Guinea Pig Polyclonal Antibody to Gephyrin
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序列
- (C)RDAIVKVKEV HDE
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预测反应
- Mouse,human - identical
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产品特性
- Guinea Pig Anti-Gephyrin Antibody (ABIN7581882) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Gephyrin from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-Gephyrin Antibody (ABIN7043214, ABIN7044520 and ABIN7044521) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation and more.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
- (C)RDAIVKVKEVHDE, corresponding to amino acid residues 183-195 of rat Gephyrin
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亚型
- IgG
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anti-Gephyrin (GPHN) antibody
GPHN 适用: 人, 猴 WB, IHC, FACS, IF, IHC (p) 宿主: 小鼠 Monoclonal 3B6 unconjugated
anti-Gephyrin (GPHN) (AA 148-736) antibodyGPHN 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Gephyrin (GPHN) antibodyGPHN 适用: 人, 大鼠, 猴 WB, FACS 宿主: 小鼠 Monoclonal 3A6 unconjugated
anti-Gephyrin (GPHN) antibodyKD Validated GPHN 适用: 人 WB, FACS 宿主: 小鼠 Monoclonal 24GB5580 unconjugated
anti-Gephyrin (GPHN) antibodyKD Validated GPHN 适用: 人 WB, FACS 宿主: 小鼠 Monoclonal 24GB5585 unconjugated
anti-Gephyrin (GPHN) (AA 437-466) antibodyGPHN 适用: 人, 小鼠 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB24625 unconjugated
anti-Gephyrin (GPHN) antibodyGPHN 适用: 人, 小鼠, 大鼠 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Gephyrin (GPHN) (AA 677-769) antibodyGPHN 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Gephyrin (GPHN) (AA 360-460) antibodyGPHN 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Gephyrin (GPHN) (AA 520-769) antibodyGPHN 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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WB: 1:200
FC: The optimal concentration should be determined by the user
ICC: The optimal concentration should be determined by the user
IHC: 1:1000
IP: The optimal concentration should be determined by the user
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说明
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Negative Control: (ABIN7235588)
Blocking Peptide: (ABIN7235588)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.
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浓度
- 0.8 mg/mL
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缓冲液
- PBS pH 7.4, 1 % BSA with 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
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- Gephyrin (GPHN)
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别名
- GPHN
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背景
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Synonyms: GPHN, Putative glycine receptor-tubulin linker protein
Description: Gephyrin is a highly conserved, intracellular scaffolding protein responsible for the post-synaptic anchoring and assembly of the inhibitory glycine and γ-aminobutyric acid type A receptors (GlyR and GABAAR), it essentially anchors the receptors, by auto-oligomerization, to the cytoskeleton1,2.GABAergic transmission is important for the mediation of neuronal excitability and has a crucial role in neurodevelopment, throughout the central and peripheral nervous systems (especially via GABAAR). Glycinergic neurons on the other hand control motor functions and sensory signaling as well as take part in pain transmission in the spine's dorsal horn. Importantly, gephyrin has a 1:1 stoichiometry with GlyR3,4.Up-to-date atomic force microscopy data of gephyrin has shown that it forms trimers with multiple conformations. It is a modular construct with three domains: an N-terminal G-, a central C- and a C-terminal E-domain. Recent work suggests also that gephyrin regulates neuronal differentiation by anchoring small cytoskeletal-related signaling molecules at unique subcellular locations2,5,6.
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基因ID
- 64845
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UniProt
- Q03555
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途径
- Synaptic Membrane, Skeletal Muscle Fiber Development
抗原
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