GAP43 抗体 (AA 205-218)
Quick Overview for GAP43 抗体 (AA 205-218) (ABIN7581879)
抗原
See all GAP43 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 205-218
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原理
- A Guinea Pig Polyclonal Antibody to GAP43
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序列
- (C)KPKESARQDE GKED
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特异性
- C-terminus
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预测反应
- Mouse - identical, human - 13 out of 14 amino acid residues identical
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产品特性
- Guinea Pig Anti-GAP43 Antibody (ABIN7581879) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GAP43 from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-GAP43 Antibody (ABIN7043263, ABIN7044662 and ABIN7044663) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation and more.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
- (C)KPKESARQDEGKED, corresponding to amino acid residues 205-218 of rat GAP43
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亚型
- IgG
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300
Application Dilutions Western blot wb: 1:400
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说明
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Negative Control: (ABIN7235735)
Blocking Peptide: (ABIN7235735)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 0.2 mL double distilled water (DDW).
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- GAP43 (Growth Associated Protein 43 (GAP43))
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别名
- GAP43
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背景
- Axonal membrane protein GAP-43, Growth-associated protein 43, Neuromodulin, Neural phosphoprotein B-50,Growth associated protein 43 (GAP43) is a neuron specific phosphoprotein that plays an important role in the process of nerve growth, development, regeneration and axonal remodeling. GAP43 belongs to the calmodulin-binding protein family and is highly distributed in the presynaptic membrane1.GAP43 is expressed in developing neurons, especially in the growth cone. In mature neurons, GAP43 is detected in regenerating nerves. In brain injury, neurons exhibit sprouting of the lateral branches and responsive regeneration of the axon accompanied by increased expression of GAP43. GAP43 expression is used as an important tool for evaluating axon injury and the regenerative response in the mature central nervous system (CNS)1,2.
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基因ID
- 29423
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UniProt
- P07936
抗原
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