ENPP1 抗体 (Extracellular)
Quick Overview for ENPP1 抗体 (Extracellular) (ABIN7581869)
抗原
See all ENPP1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 711-725, Extracellular
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原理
- A Rabbit Polyclonal antibody to ENPP1 (extracellular)
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序列
- CLYQDFRIPL SPVHK
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特异性
- Extracellular, C-term.
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预测反应
- Mouse - 14 out of 15 amino acid residues identical, Rat- 13 out of 15 amino acid residues identical
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产品特性
- Anti-ENPP1 (extracellular) Antibody (ABIN7581869) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize ENPP1 from mouse, rat and human samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
- CLYQDFRIPLSPVHK, corresponding to amino acid residues 711 - 725 of human ENPP1
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亚型
- IgG
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anti-Ectonucleotide Pyrophosphatase/phosphodiesterase 1 (ENPP1) (C-Term) antibody
Verified ENPP1 适用: 人 IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Ectonucleotide Pyrophosphatase/phosphodiesterase 1 (ENPP1) antibodyKO Validated ENPP1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Ectonucleotide Pyrophosphatase/phosphodiesterase 1 (ENPP1) (AA 568-793) antibodyENPP1 适用: 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ectonucleotide Pyrophosphatase/phosphodiesterase 1 (ENPP1) (AA 6-34), (N-Term) antibodyENPP1 适用: 人 WB 宿主: 兔 Polyclonal RB41733 unconjugated
anti-Ectonucleotide Pyrophosphatase/phosphodiesterase 1 (ENPP1) (AA 1-873) antibodyENPP1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Ectonucleotide Pyrophosphatase/phosphodiesterase 1 (ENPP1) (AA 251-350) antibodyENPP1 适用: 小鼠, 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Ectonucleotide Pyrophosphatase/phosphodiesterase 1 (ENPP1) (AA 41-140) antibodyENPP1 适用: 小鼠 WB, ELISA, ICC, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Ectonucleotide Pyrophosphatase/phosphodiesterase 1 (ENPP1) (AA 362-518) antibodyENPP1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ectonucleotide Pyrophosphatase/phosphodiesterase 1 (ENPP1) (AA 1-75) antibodyENPP1 适用: 人 ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Ectonucleotide Pyrophosphatase/phosphodiesterase 1 (ENPP1) (AA 29-57), (N-Term) antibodyENPP1 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal RB30657 unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:1000
Application Dilutions Western blot wb: 1:200-1:400
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 0.2 mL double distilled water (DDW).
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- ENPP1 (Ectonucleotide Pyrophosphatase/phosphodiesterase 1 (ENPP1))
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别名
- ENPP1
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背景
- Ectonucleotide Pyrophosphatase/ Phosphodiesterase Family Member 1, Plasma-Cell Membrane Glycoprotein PC-1, CD203a, Membrane Component Chromosome 6 Surface Marker 1, M6S1,Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1), also known as Plasma-Cell Membrane Glycoprotein (PC-1) and CD203a, is a member the ecto-enzyme ENPP family that includes seven members (ENPP1-ENPP7)1.ENPP1 is involved in purinergic signaling as it catalyzes the hydrolysis of ATP or GTP to AMP or GMP, while generating inorganic pyrophosphates (PPi)1,2. Inorganic pyrophosphates inhibit bone and cartilage mineralization, hence making ENPP1 a key regulator of bone and cartilage development and remodeling 2,3. Indeed, several hereditary mineralization or calcification-related disorders, have been linked to loss-of-function mutations of ENPP1, such as general arterial calcification of infancy (GACI) or Cole disease 3.Recently, an intriguing new role of ENPP1 as a central regulator of immunity and tumor proliferation came to light. It was shown that ENPP1 is the critical enzyme that degrades the stimulator of interferon genes (STING) ligand, cyclic GMP-AMP (cGAMP)4,5. cGAMP was first described as an intracellular second messenger, synthesized by the enzyme cyclic-GMP-AMP synthase (cGAS) in response to self and pathogenic double-stranded DNA in the cytosol. Intracellular signaling of cGAMP is carried through the STING pathway which then directs several antiviral and anticancer cellular response mechanisms 4,5.cGAMP can also be released to the extracellular space where it can be transported into cells by receptors like MERTK 6, P2X7 6, SLC19A1 7 and LRRC8A 8. ENPP1 is the only known ecto-enzyme that can inactivate extracellular cGAMP to GMP and AMP, and thus functions as an inhibitor of the anti-tumor response elicited by extracellular cGAMP 4,9.
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基因ID
- 5167
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UniProt
- P22413
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途径
- Regulation of Carbohydrate Metabolic Process
抗原
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