CARTPT 抗体 (Secreted)
Quick Overview for CARTPT 抗体 (Secreted) (ABIN7581841)
抗原
See all CARTPT 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 95-108, Secreted
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原理
- A Rabbit Polyclonal antibody to CART peptide
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序列
- CDAGEQ(S)AVRKGAR
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特异性
- Secreted
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交叉反应 （详细）
- The antibody will recognize the precursor (proCART) and both active CART peptides: CART (55-102) and CART (62-102). The human counterpart called CART (42-89) is identical to CART (55-102).
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预测反应
- Mouse,rat,human - 13 out of 14 amino acid residues identical
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产品特性
- Anti-CART Antibody (ABIN7581841) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize CART peptide from rat, mouse and human samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
- CDAGEQ(S)AVRKGAR, corresponding to amino acid residues 95 - 108 of mouse CARTPT
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亚型
- IgG
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anti-CART Prepropeptide (CARTPT) antibody
CARTPT 适用: 人, 大鼠, 小鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-CART Prepropeptide (CARTPT) (AA 1-116) antibodyCARTPT 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3E4 unconjugated
anti-CART Prepropeptide (CARTPT) antibodyCARTPT 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-CART Prepropeptide (CARTPT) (AA 28-116) antibodyCARTPT 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-CART Prepropeptide (CARTPT) (AA 28-129) antibodyCARTPT 适用: 大鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C4 unconjugated
anti-CART Prepropeptide (CARTPT) (AA 28-129) antibodyCARTPT 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CART Prepropeptide (CARTPT) (AA 26-55), (N-Term) antibodyCARTPT 适用: 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB31281 unconjugated
anti-CART Prepropeptide (CARTPT) (AA 25-55), (N-Term) antibodyCARTPT 适用: 人, 小鼠 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300
Application Dilutions Western blot wb: 1:200
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 0.2 mL double distilled water (DDW).
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- CARTPT (CART Prepropeptide (CARTPT))
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别名
- CARTPT
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背景
- Cocaine-And Amphetamine-Regulated Transcript Protein, CARTPT, CART Prepropeptide,CART peptide, known by the name CARTPT - Cocaine-And Amphetamine-Regulated Transcript Protein. CART peptide is a satiety factor closely associated with the actions of leptin and neuropeptide Y. This anorectic peptide inhibits both normal and starvation-induced feeding and completely blocks the feeding response induced by neuropeptide Y (NPY) and regulated by leptin in the hypothalamus. It promotes neuronal development and survival in vitro1.CART peptide is a widely distributed neurotransmitter expressed in the central nervous systems, with a role in reward and reinforcement, feeding, development, sensory processing, stress, and endocrine control. Expression of CART peptide dominates in the mesocorticolimbic dopaminergic (DA) system that extends from the ventral tegmental area (VTA) to the nucleus accumbens (NAc) and includes other limbic areas (amygdala, hippocampus, and frontal cortex), and is also widely distributed in the central nervous system (CNS)2.CART peptide exerted inhibitory effect on psychostimulant-enhanced dopamine receptors signaling, Ca2+/calmodulin-dependent kinase signaling and crucial transcription factors expression. Besides modulation of dopamine receptors-related pathways, CART peptide also exhibited elaborated interactions with other neurotransmitter receptors, such as glutamate receptors and γ-aminobutyric acid receptors, which further account for attribution of CART peptide to inhibition of psychostimulant-potentiated locomotor activity. CART peptide has been shown to have anxiolytic functions on the aversive mood and uncontrolled drug-seeking behaviors following drug withdrawal. Moreover, microinjection of CART peptide has been shown to have an anti-depressant effect, which suggests its potential utility in the mood regulation and avoidance of depression-like behaviors3.
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基因ID
- 27220
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UniProt
- P56388
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途径
- Hormone Transport, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Feeding Behaviour
抗原
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