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GFAP 抗体 (AA 51-150)

Name: GFAP 抗体 (AA 51-150)
SKU: ABIN726200
Availability: OutOfStock

This anti-GFAP antibody is a 兔多克隆 antibody detecting GFAP in WB, ELISA, ICC, FACS, IHC (p), IHC (fro), IF (p) 和 IF (cc). Suitable for 人, 大鼠和小鼠. This Primary Antibody has been cited in 20+ publications.

U251 Cells lysates probed with GFAP Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C. (GFAP 抗体 (AA 51-150))

Image provided by One World Lab validation program. (GFAP 抗体 (AA 51-150))

IF (cc)

Formalin-fixed and paraffin embedded: rat brain tissue Anti-GFAP Polyclonal Antibody, Unconjugated at 1:400, followed by conjugation to the secondary antibody and DAB staining (GFAP 抗体 (AA 51-150))

IHC (p)

Paraformaldehyde-fixed, paraffin embedded rat brain, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30min, Antibody incubation with GFAP Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated secondary -AF594) at 1:500 for 90 minutes and DAPI staining of the nuclei. (GFAP 抗体 (AA 51-150))

8 images

(20 references)

产品编号 ABIN726200

发货至: 中国

Datasheet as PDF

Quick Overview for GFAP 抗体 (AA 51-150) (ABIN726200)

抗原

See all GFAP 抗体

GFAP (Glial Fibrillary Acidic Protein (GFAP))

适用

人, 大鼠, 小鼠

宿主

兔

克隆类型

多克隆

标记

This GFAP antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

抗原表位
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AA 51-150

交叉反应

人, 小鼠, 大鼠

预测反应

Dog,Cow,Sheep,Pig,Rabbit

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human GFAP

亚型

IgG
anti-Glial Fibrillary Acidic Protein (GFAP) (C-Term) antibody
GFAP 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Glial Fibrillary Acidic Protein (GFAP) (AA 93-432) antibody
GFAP 适用: 人, 大鼠, 小鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Glial Fibrillary Acidic Protein (GFAP) (AA 253-354) antibody
GFAP 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated

anti-Glial Fibrillary Acidic Protein (GFAP) (AA 1-430) antibody
GFAP 适用: 大鼠 WB, IHC, ICC, IP 宿主: 小鼠 Monoclonal C1 unconjugated

anti-Glial Fibrillary Acidic Protein (GFAP) (AA 292-432) antibody
GFAP 适用: 人 WB, IHC, IF, ELISA, FACS 宿主: 小鼠 Monoclonal 1C91F1 unconjugated

anti-Glial Fibrillary Acidic Protein (GFAP) (pSer38) antibody
GFAP 适用: 人, 大鼠, 小鼠 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated

anti-Glial Fibrillary Acidic Protein (GFAP) (C-Term) antibody
Verified GFAP 适用: 人, 大鼠, 小鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated

anti-Glial Fibrillary Acidic Protein (GFAP) antibody
GFAP 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated

anti-Glial Fibrillary Acidic Protein (GFAP) (AA 108-211) antibody
GFAP 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated

anti-Glial Fibrillary Acidic Protein (GFAP) antibody
GFAP 适用: 人, 大鼠, 小鼠, Pig, Cow, 小鸡, 兔 WB, IHC, IF, FACS, StM 宿主: 小鼠 Monoclonal GA-5 unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Farah, Siwek, Cummings: "Tau accumulations in the brains of woodpeckers." in: PLoS ONE, Vol. 13, Issue 2, pp. e0191526, (2018) (PubMed).

Liao, Xiao, Zhu, Zhou: "The effect of celastrol on learning and memory in diabetic rats after sevoflurane inhalation." in: Archives of medical science : AMS, Vol. 14, Issue 2, pp. 370-380, (2018) (PubMed).

Hu, Kodithuwakku, Zhou, Li, Han, Fang, Liu, Li: "Levo-Corydalmine Alleviates Neuropathic Cancer Pain Induced by Tumor Compression via the CCL2/CCR2 Pathway." in: Molecules (Basel, Switzerland), Vol. 22, Issue 6, (2017) (PubMed).

Sweeney, Qi, Xu, Yang: "Activation of hypothalamic astrocytes suppresses feeding without altering emotional states." in: Glia, Vol. 64, Issue 12, pp. 2263-2273, (2016) (PubMed).

Yan, Li, Li, Lin, Yang: "Osthole Protects Bone Marrow-Derived Neural Stem Cells from Oxidative Damage through PI3K/Akt-1 Pathway." in: Neurochemical research, (2016) (PubMed).

Zhao, Sun, Miao, Ji, Zhao, Sun, Miao, Yoshii, Mizushima, Wang, Zhang: "The autophagy gene Wdr45/Wipi4 regulates learning and memory function and axonal homeostasis." in: Autophagy, Vol. 11, Issue 6, pp. 881-90, (2015) (PubMed).

Mori, Matsubara, Matsubara, Uchikura, Hashimoto, Fujioka, Matsumoto: "Stromal Cell-Derived Factor-1? Plays a Crucial Role Based on Neuroprotective Role in Neonatal Brain Injury in Rats." in: International journal of molecular sciences, Vol. 16, Issue 8, pp. 18018-32, (2015) (PubMed).

Shan, Huang, You, Yuan, Gao, Hu, Du: "High efficiency intracellular transport of cationic peptide stearate for gene delivery in tumor cells and multipotent stem cells." in: Journal of biomedical nanotechnology, Vol. 10, Issue 11, pp. 3231-43, (2015) (PubMed).

Liang, Sun, Yu, He, Xie: "Scorpion ethanol extract and valproic acid effects on hippocampal glial fibrillary acidic protein expression in a rat model of chronic-kindling epilepsy induced by lithium chloride-pilocarpine." in: Neural regeneration research, Vol. 7, Issue 6, pp. 426-33, (2015) (PubMed).

Fan, Hu, Ji, Sun, Xiong, Yang, Liu: "Directed differentiation of aged human bone marrow multipotent stem cells effectively generates dopamine neurons." in: In vitro cellular & developmental biology. Animal, Vol. 50, Issue 4, pp. 304-12, (2014) (PubMed).

Xiang, Wei, Chen, Liu, Liu, Wang, Zhang: "Anti-inflammatory effect of acetylpuerarin on eicosanoid signaling pathway in primary rat astrocytes." in: Journal of molecular neuroscience : MN, Vol. 52, Issue 4, pp. 577-85, (2014) (PubMed).

Ma, Cao, Li, Gao, Qi, Zhao, Du, Xue, Peng, Wen, Chen, Ning, Huang, Zhang, Gao, Yu, Chen: "Dapper1 promotes autophagy by enhancing the Beclin1-Vps34-Atg14L complex formation." in: Cell research, Vol. 24, Issue 8, pp. 912-24, (2014) (PubMed).

Du, Liu, Chen, Dou, Lei, Chang, Cai, Cui, Yang, Sun, Li, Jiang: "Targeting the SMO oncogene by miR-326 inhibits glioma biological behaviors and stemness." in: Neuro-oncology, (2014) (PubMed).

Zuo, Wang, Li, Lin, Duan, Qi, Li, Sun, Wu: "Existence of glia mitigated ketamine-induced neurotoxicity in neuron-glia mixed cultures of neonatal rat cortex and the glia-mediated protective effect of 2-PMPA." in: Neurotoxicology, Vol. 44, pp. 218-30, (2014) (PubMed).

Hu, Zheng, Jia, Li, Dong, Wang, Lin, Zhang, Zhao, Xu, Zhou, Yuan, Zhang, Ren: "Neuroprotection effect of interleukin (IL)-17 secreted by reactive astrocytes is emerged from a high-level IL-17-containing environment during acute neuroinflammation." in: Clinical and experimental immunology, Vol. 175, Issue 2, pp. 268-84, (2014) (PubMed).

Zhao, Zhao, Wang, Xu, Miao, Feng, Chen, Kovács, Fan, Zhang: "Mice deficient in Epg5 exhibit selective neuronal vulnerability to degeneration." in: The Journal of cell biology, Vol. 200, Issue 6, pp. 731-41, (2013) (PubMed).

Liu, Xue, Tang, Hou, Qi, Chen, Chen, Zhang, Chen, Xu: "A simple method for isolating and culturing the rat brain microvascular endothelial cells." in: Microvascular research, Vol. 90, pp. 199-205, (2013) (PubMed).

Xiang, Chen, Liu, Liu, Wei, Sun, Wang, Zhang: "Inhibition of sPLA?-IIA prevents LPS-induced neuroinflammation by suppressing ERK1/2-cPLA?? pathway in mice cerebral cortex." in: PLoS ONE, Vol. 8, Issue 10, pp. e77909, (2013) (PubMed).

Zhao, Zhao, Miao, Wang, Sun, Zhang: "The p53-induced gene Ei24 is an essential component of the basal autophagy pathway." in: The Journal of biological chemistry, Vol. 287, Issue 50, pp. 42053-63, (2012) (PubMed).
抗原

GFAP (Glial Fibrillary Acidic Protein (GFAP))

别名

GFAP

背景

Synonyms: Glial fibrillary acidic protein, GFAP
Background: GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

基因ID

2670

UniProt

P14136