Iba1 抗体 (Intracellular)
Quick Overview for Iba1 抗体 (Intracellular) (ABIN7581823)
抗原
See all Iba1 (IBA1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 40-53, Intracellular
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原理
- A Guinea Pig Polyclonal Antibody to IBA1/AIF1
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序列
- (C)DEDLQSKLEA FKTK
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特异性
- Intracellular
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预测反应
- Mouse - 13 out of 14 amino acid residues identical,human - 11 out of 14 amino acid residues identical
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产品特性
- Guinea Pig Anti-IBA1/AIF1 Antibody (ABIN7581823) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize IBA1/AIF1 from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-IBA1/AIF1 Antibody (ABIN7042910, ABIN7044214 and ABIN7044215) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation and more.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
- (C)DEDLQSKLEAFKTK, corresponding to amino acid residues 40-53 of rat IBA1/AIF1
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亚型
- IgG
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anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (C-Term) antibody
IBA1 适用: 人 WB, IHC, ICC, IF, IHC (p), FACS, IHC (fro), IHC (ff) 宿主: 兔 Polyclonal unconjugated
anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (AA 51-147) antibodyIBA1 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) antibodyIBA1 适用: 人 WB, IHC, IP, ICC, IF, IHC (p), FACS, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (AA 1-147) antibodyIBA1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (AA 1-147) antibodyIBA1 适用: 大鼠 WB 宿主: 小鼠 Monoclonal C5 unconjugated
anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (C-Term) antibodyVerified IBA1 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (Internal Region) antibodyVerified IBA1 适用: 人 IHC, ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated
anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (AA 1-147) antibodyIBA1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (AA 35-102) antibodyIBA1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (AA 1-146) antibodyIBA1 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal AIF1-1909 unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:1000
Application Dilutions Western blot wb: 1:200
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说明
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Negative Control: (ABIN7234692)
Blocking Peptide: (ABIN7234692)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 0.2 mL double distilled water (DDW).
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))
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别名
- AIF1
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背景
- Ionized calcium-binding adapter molecule 1, Allograft Inflammatory Factor 1, MRF-1, Microglia response factor,Allograft Inflammatory Factor-1 (AIF1) is a 17 kDa. cytoplasmic, calcium and actin binding scaffold protein, mainly expressed in immunocytes. AIF-1 is induced by cytokines such as interferon-γ (IFN-γ).It is encoded within the HLA class III genomic region by IBA1 gene, and its structure contains seven alpha helixes and three beta sheets. There are three other proteins identical to AIF1: Ionized Ca2+-Binding Adaptor 1, Microglia Response Factor-1, and Daintain1.The gene is highly expressed in testis, spleen, circulating macrophages and in the brain but weakly expressed in lung and kidney. Among brain cells, the Iba1 gene is strongly and specifically expressed in microglia and its expression is up-regulated following nerve injury, central nervous system ischemia, and several other brain diseases2.AIF1 influences the immune system at several key points and thus modulates inflammatory diseases. It increases the expression of inflammatory mediators such as cytokines, chemokines, inducible nitric oxide synthase (iNOS) and promotes inflammatory cell proliferation and migration and hence it is highly associated with autoimmune diseases3. In addition, its abundance is positively correlated with metabolic indicators, such as body mass index, triglycerides, and fasting plasma glucose levels. There is also evidence that AIF1 could be a marker for diabetic nephropathy when detected in serum. It is found in activated macrophages in the pancreatic islets, and has been shown to decrease insulin secretion, while simultaneously impairing glucose elimination4.
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基因ID
- 29427
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UniProt
- P55009
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途径
- Smooth Muscle Cell Migration
抗原
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