Recombinant SARS-CoV-2 Spike 抗体 (AA 319-541)
Quick Overview for Recombinant SARS-CoV-2 Spike 抗体 (AA 319-541) (ABIN7581775)
抗原
See all SARS-CoV-2 Spike 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
质量等级
克隆位点
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抗原表位
- AA 319-541
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Expression System
- Phage display
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原理
- recombinant anti-SARS-CoV-2 spike antibody
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特异性
- This is an antibody developed by antibody phage display technology using a human naive antibody gene library and SARS-CoV-2 antigen. For this antibody both the heavy and light chains are cloned and expressed, generating full-length antibodies.
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交叉反应 （详细）
- No known cross reactivity. No cross-reactivity to other human coronaviruses.
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产品特性
- This antibody can be detected with anti-mouse Fc secondary antibodies.
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纯化方法
- Protein A purification
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免疫原
- No immunization, animal-free antibody development. Antigen: Spike Protein SARS-CoV2 RVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNF
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亚型
- IgG2a
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anti-SARS-CoV-2 Spike (C-Term) antibody
适用: SARS Coronavirus-2 (SARS-CoV-2), SARS Coronavirus (SARS-CoV) ELISA, WB, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-SARS-CoV-2 Spike antibody适用: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Beta, SARS CoV-2 Gamma, SARS CoV-2 Alpha ELISA, WB 宿主: 兔 Polyclonal unconjugated
anti-SARS-CoV-2 Spike antibody适用: SARS Coronavirus-2 (SARS-CoV-2) ELISA, WB 宿主: Human Monoclonal AM004414 (414-4) unconjugated Recombinant Antibody
anti-SARS-CoV-2 Spike antibody适用: SARS Coronavirus-2 (SARS-CoV-2) ELISA 宿主: Human Monoclonal AM015553 (553-15) unconjugated Recombinant Antibody
anti-SARS-CoV-2 Spike (AA 319-541) antibody适用: SARS Coronavirus-2 (SARS-CoV-2) ELISA, FACS 宿主: 兔 Monoclonal DM54 unconjugated Recombinant Antibody
anti-SARS-CoV-2 Spike (RBD) antibody适用: SARS Coronavirus-2 (SARS-CoV-2) WB 宿主: 山羊 Polyclonal unconjugated
anti-SARS-CoV-2 Spike antibodyResearch Grade 适用: SARS Coronavirus-2 (SARS-CoV-2) ELISA, FACS 宿主: Human Monoclonal CR3022 unconjugated Recombinant Antibody
anti-SARS-CoV-2 Spike (RBD) antibodyVerified 适用: SARS Coronavirus-2 (SARS-CoV-2) ELISA, WB 宿主: 山羊 Polyclonal unconjugated
anti-SARS-CoV-2 Spike (RBD) antibody适用: SARS Coronavirus-2 (SARS-CoV-2) ELISA, FACS 宿主: 兔 Monoclonal DM26 unconjugated
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应用备注
- Western Blot: 0.2-5 μg/mL ELISA: 1-12 μg/mL as coating antibody 0.5-5 μg/mL as detection antibody IF: 0.1 - 10 μg/mL Optimal working dilution should be determined by the investigator
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS, pH 7.4,
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储存条件
- -20 °C
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- SARS-CoV-2 Spike
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别名
- SARS-CoV-2 spike protein (RBD)
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背景
- Coronaviruses (CoVs) are enveloped non-segmented positive-sense single-stranded RNA viruses and can infect respiratory, gastrointestinal, hepatic and central nervous system of human and many other wild animals. Recently, a new severe acute respiratory syndrome β-coronavirus called SARS-CoV-2 (or 2019-nCoV) has emerged, which causes an epidemic of acute respiratory syndrome (called coronavirus human disease 2019 or COVID-19). SARS-CoV-2 shares 79.5 % sequence identity with SARS-CoV and is 96.2 % identical at the genome level to the bat coronavirus BatCoV RaTG133, suggesting it had originated in bats. SARS-CoV-2 contains 4 structural proteins, including Envelope (E), Membrane (M), Nucleocapsid (N) and Spike (S), which is a transmembrane protein, composed of two subunits S1 and S2. The S protein plays a key role in viral infection and pathogenesis. The S1 subunit contains the N-terminal domain (NTD) and a receptor binding domain (RBD), which binds to the cell surface receptor Angiotensin-Converting Enzyme 2 (ACE2) present at the surface of epithelial cells, causing mainly infection of human respiratory cells, whereas S2 harbors heptad repeat 1 (HR1) and HR2. The RBD domain first binds its receptor to form an RBD/ACE2 complex. This triggers conformational changes in the S protein, leading to membrane fusion mediated via HR1 and HR2 and consequently in viral entry into target cells. Antibodies targeting various regions of S protein have different mechanisms in inhibiting SARS-CoV-2 infection. For example, NTD-targeting antibodies bind the NTD to form an NTD/mAb complex, thereby preventing conformational changes in the S protein and blocking membrane fusion and viral entry. RBD-targeting antibodies form RBD/mAb or RBD/Nb complexes that could inhibit binding of the RBD to ACE2, thereby preventing entry of SARS-CoV-2 into target cells.
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分子量
- 76 kDa
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NCBI登录号
- YP_009724390
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UniProt
- P0DTC2
抗原
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