Recombinant Myc 标记 抗体 (AA 408-439)
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- 抗原 See all Myc Tag products
- Myc Tag
- 抗体类型
- Recombinant Antibody
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抗原表位
- AA 408-439
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适用
- 标记
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宿主
- 兔
- Expression System
- Phage display
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克隆类型
- 单克隆
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标记
- This Myc Tag antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
- 原理
- Rabbit Anti-c-myc Antibody, animal-free mAb
- 特异性
- This is an antibody originating from 9E10 hybridoma clone. Improved via phage display technology. Affinity and thermal stability improved over original mouse IgG clone 9E10
- 交叉反应 (详细)
- No known cross reactivity. No binding to native c-Myc protein.
- 产品特性
- This antibody can be detected with anti-rabbit Fc secondary antibodies.
- 纯化方法
- Protein A purification
- 质量等级
- Animal-Free
- 免疫原
- No immunization, animal-free antibody development. Origin of original 9E10 clone: A synthetic peptide corresponding to aa 408-439 from C-terminus of human c-myc
- 克隆位点
- TUN219-2C1 (9E10)
- 亚型
- IgG
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- 应用备注
- Western Blot: 0.2-5 μg/mL ELISA: 1-12 μg/mL as coating antibody 0.5-5 μg/mL as detection antibody IF: 0.1 - 10 μg/mL Optimal working dilution should be determined by the investigator
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- PBS, pH 7.4,
- 储存条件
- -20 °C
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- 抗原
- Myc Tag
- 别名
- c-myc tag (Myc Tag 产品)
- 物质类
- Tag
- 背景
- Synthetic peptide of human c-Myc, aa408-439. The c-Myc or myc-tag is widely used to detect the expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems. Hyper-Myc is an improved engineered anti c-Myc Tag antibody. It shows a higher affinity and stability in direct comparison to hybridoma antibody 9E10 that shares the same epitope. It is further thoroughly characterized by cross-reactivity profiling on 28895 human epitopes showing higher specificity and increased monovalent affinity.
- 分子量
- 1.2 kDa
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