Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
原理
Rabbit Anti-c-myc Antibody, animal-free mAb
特异性
This is an antibody originating from 9E10 hybridoma clone. Improved via phage display technology. Affinity and thermal stability improved over original mouse IgG clone 9E10
交叉反应 (详细)
No known cross reactivity. No binding to native c-Myc protein.
产品特性
This antibody can be detected with anti-rabbit Fc secondary antibodies.
纯化方法
Protein A purification
质量等级
Animal-Free
免疫原
No immunization, animal-free antibody development. Origin of original 9E10 clone: A synthetic peptide corresponding to aa 408-439 from C-terminus of human c-myc
Western Blot: 0.2-5 μg/mL ELISA: 1-12 μg/mL as coating antibody 0.5-5 μg/mL as detection antibody IF: 0.1 - 10 μg/mL Optimal working dilution should be determined by the investigator
Synthetic peptide of human c-Myc, aa408-439. The c-Myc or myc-tag is widely used to detect the expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems. Hyper-Myc is an improved engineered anti c-Myc Tag antibody. It shows a higher affinity and stability in direct comparison to hybridoma antibody 9E10 that shares the same epitope. It is further thoroughly characterized by cross-reactivity profiling on 28895 human epitopes showing higher specificity and increased monovalent affinity.