Recombinant Myc 标记 抗体 (AA 408-439)
Quick Overview for Recombinant Myc 标记 抗体 (AA 408-439) (ABIN7581766)
抗原
抗体类型
适用
宿主
克隆类型
标记
应用范围
质量等级
克隆位点
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抗原表位
- AA 408-439
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Expression System
- Phage display
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原理
- Mouse Anti-c-myc Antibody, animal-free mAb
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特异性
- This is an antibody originating from 9E10 hybridoma clone. Improved via phage display technology. Affinity and thermal stability improved over original mouse IgG clone 9E10
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交叉反应 （详细）
- No known cross reactivity. No binding to native c-Myc protein.
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产品特性
- This antibody can be detected with anti-mouse Fc secondary antibodies.
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纯化方法
- Protein A purification
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免疫原
- No immunization, animal-free antibody development. Origin of original 9E10 clone: A synthetic peptide corresponding to aa 408-439 from C-terminus of human c-myc
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亚型
- IgG2a
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anti-Myc Tag (AA 408-439) antibody
Animal-Free 适用: 标记 WB, ELISA, IF, FACS 宿主: 兔 Monoclonal TUN219-2C1 (9E10) unconjugated Recombinant Antibody
anti-Myc Tag antibodyWB, IP, ELISA, IF 宿主: 小鼠 Monoclonal unconjugated
anti-Myc Tag (AA 408-439) antibodyAnimal-Free 适用: 标记 WB, ELISA, IF, FACS 宿主: Human Monoclonal TUN219-2C1 (9E10) unconjugated Recombinant Antibody
anti-Myc Tag (C-Term) antibody适用: 人 WB, IP, ELISA, IHC (fro), IHC (p), ICC, FACS, ICFC 宿主: 小鼠 Monoclonal 9E10 unconjugated
anti-Myc Tag (C-Term) antibody (Biotin)适用: 人 WB, IP, ELISA, IHC (fro), IHC (p), ICC, FACS, ICFC 宿主: 小鼠 Monoclonal 9E10 Biotin
anti-Myc Tag (C-Term) antibody (FITC)适用: 人 FACS, ICFC 宿主: 小鼠 Monoclonal 9E10 FITC
anti-Myc Tag antibodyWB, IP, ELISA, IF 宿主: 小鼠 Monoclonal 2G8D5 unconjugated
anti-Myc Tag (AA 408-439) antibodyWB, ELISA 宿主: 小鼠 Monoclonal 9E10- unconjugated
anti-Myc Tag (AA 410-419) antibodyWB 宿主: 山羊 Polyclonal unconjugated
anti-Myc Tag (AA 408-439), (C-Term) antibodyIF, IHC, FACS, StM 宿主: 小鼠 Monoclonal 9E10-3 unconjugated
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应用备注
- Western Blot: 0.2-5 μg/mL ELISA: 1-12 μg/mL as coating antibody 0.5-5 μg/mL as detection antibody IF: 0.1 - 10 μg/mL Optimal working dilution should be determined by the investigator
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS, pH 7.4,
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储存条件
- -20 °C
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- Myc Tag
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别名
- c-myc tag
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物质类
- Tag
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背景
- Synthetic peptide of human c-Myc, aa408-439. The c-Myc or myc-tag is widely used to detect the expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems. Hyper-Myc is an improved engineered anti c-Myc Tag antibody. It shows a higher affinity and stability in direct comparison to hybridoma antibody 9E10 that shares the same epitope. It is further thoroughly characterized by cross-reactivity profiling on 28895 human epitopes showing higher specificity and increased monovalent affinity.
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分子量
- 1.2 kDa
抗原
-
