Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)
原理
Rabbit polyclonal antibody to human Vac14 with reactivity to mouse/rat and human Vac14
特异性
The Vac14 antibody is directed at an C-terminal region of Vac14 and detects mammalian forms of Vac14 with an expected molecular weight of approximately 82-88 kDa.
交叉反应
小鼠, 大鼠
纯化方法
affinity purified using Protein A chromatography
免疫原
The antibody was generated from a fusion protein with amino acids 523 to 782 of human Vac14.
IF/ICC – 2-5 ug/mL, Western – 1.0 ug/mL, Immunoprecipitation – 1.0 ug/mL Other in vitro and cellular applications are possible using this antibody, but have not been verified.
限制
仅限研究用
状态
Liquid
缓冲液
PBS, pH 7.4
储存条件
4 °C,-80 °C
储存方法
protect from direct light and store at 4°C for up to 30 days. For long term storage, aliquot and store at -80°C. Avoid repeated freeze/thaw cycles.
Vac14 is a scaffold protein that functions in a complex with PIKfyve and Sac3 (FIG4) to regulate the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PI(3,5)P2) on endosomal membranes. The coordinated activity within the complex maintains homeostasis of PI(3)P and PI(5)P and plays a role in the fusion and transport of endosomes