Double-Stranded RNA (dsRNA) 抗体
This 小鼠 单克隆 antibody specifically detects Double-Stranded RNA in ELISA 和 IF.
产品编号 ABIN7567105
发货至:
中国
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Contact Info
Our Local Distributor
中国
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北京 101111
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Quick Overview for Double-Stranded RNA (dsRNA) 抗体 (ABIN7567105)
抗原
Double-Stranded RNA (dsRNA)
适用
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宿主
小鼠
克隆类型
单克隆
标记
非结合性
应用范围
ELISA, Immunofluorescence (IF)
克隆位点
K2
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原理
- Mouse anti double-stranded RNA (K2)
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特异性
- The mAb K2 recognises double-stranded RNA (dsRNA) provided that the length of the helix is greater than or equal to 40 bp dsRNA. Recognition is independent of the sequence and nucleotide composition of the antigen. All naturally occurring dsRNAs investigated up to now (4050 species) as well as poly(I).poly(C) and poly(A).poly(U) have been recognised by K2. As described by Schönborn et al. K2 binds with high avidity to all dsRNAs investigated.
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产品特性
- K2 anti-dsRNA monoclonal antibody has an IgM isotype. K2 has primarily been used in ELISA and sandwich ELISA. Generally, K2 can be used in applications where an anti-dsRNA antibody with an isotype other than IgG (IgG2a) is required.The mAb K2 recognises double-stranded RNA (dsRNA) provided that the length of the helix is greater than or equal to 40 bp dsRNA. Recognition is independent of the sequence and nucleotide composition of the antigen. All naturally occurring dsRNAs investigated up to now (4050 species) as well as poly(I).poly(C) and poly(A).poly(U) have been recognised by K2. As described by Schönborn et al. K2 binds with high avidity to all dsRNAs investigated.
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纯化方法
- Undiluted hybridoma supernatant
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免疫原
- Female DBA/2 mice were injected intraperitonially with a mixture of 50 ug L-dsRNA and 75 ug methylated bovine serum albumin, emulsified in complete Freund's adjuvant. After several boosts spleen cells were fused with Sp2/0-Agl4 myeloma cells to generate the hybridoma clone.
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亚型
- IgM kappa
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应用备注
- MAb K2 is primarily used for a sandwich ELISA to detect and quantitate (after calibration) dsRNA (see Schönborn et al.). For this application it should be diluted with PBS. It may also be advantageous to use K2 for immunofluorescence studies. The optimum working dilution of the antibody for any specific application should be established by titration. Not for use for clinical purposes. For in vitro use only.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Culture supernatant (RPMI, 5% fetal calf serum). No preservative is added.
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储存条件
- -20 °C,-80 °C
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储存方法
- After delivery antibodies should be aliquoted and stored at -20 ° or -70 °C. After adding 10 mM sodium azide undiluted antibody can also be stored at +4 °C for a short period of time. For long term storage the antibody should be kept frozen. Repeated freezing/thawing cycles should be avoided.
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- Double-Stranded RNA (dsRNA)
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别名
- dsRNA
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物质类
- Nucleotide
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背景
- Over the past decade our double-stranded RNA (dsRNA)antibodies have been used extensively to detect and characterise plant and animal viruses with dsRNA genomes or intermediates. In addition, the anti-dsRNA antibodies can be used as a diagnostic tool to detect pathogens, including detection in paraffin-embedded fixed tissue samples (Richardson et al. 2010).
抗原
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