Recombinant TNFSF13 抗体
Quick Overview for Recombinant TNFSF13 抗体 (ABIN7566472)
抗原
See all TNFSF13 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
原理
- anti-APRIL (mouse), mAb (rec.) (blocking) (Apry-1-1) (Fc LAEA-PG)
-
产品特性
-
Recombinant Antibody. Recognizes mouse APRIL. Does not recognize human APRIL and mouse/human BAFF. Source: HEK293. Isotype: Mouse IgG2blambda. Immunogen: Mouse APRIL (aa 98-232). Applications: ELISA, FUNC (Blocking), IP. Inhibits the binding of mouse APRIL to BCMA and TACI. Works in vitro and in vivo. APRIL is a cytokine that belongs to the TNF superfamily and binds to TACI and BCMA. It is implicated in the regulation of tumor cell growth and is involved in monocyte/macrophage-mediated immunological processes. anti-APRIL (mouse), mAb (rec.) (Blocking) (Apry-1-1) (Fc LAEA-PG) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc mouse portion with mutations LAEA-PG and then produced in mammalian cells to generate an IgG like scFv-Fc (LAEA-PG) fusion protein. Mutations LAEA-PG inhibit binding to FcgammaRs and C1q while FcRn binding and Fc stability remain unaffected.
APRIL is a cytokine that belongs to the TNF superfamily and binds to TACI and BCMA. It is implicated in the regulation of tumor cell growth and is involved in monocyte/macrophage-mediated immunological processes. anti-APRIL (mouse), mAb (rec.) (Blocking) (Apry-1-1) (Fc LAEA-PG) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc mouse portion with mutations LAEA-PG and then produced in mammalian cells to generate an IgG like scFv-Fc (LAEA-PG) fusion protein. Mutations LAEA-PG inhibit binding to FcgammaRs and C1q while FcRn binding and Fc stability remain unaffected.
-
内毒素水平
- <0.001EU/μg purified protein (LAL-test, Lonza)
-
免疫原
- Mouse APRIL (aa 98-232).
-
亚型
- IgG2b lambda
-
Biological Activity Comment
- Inhibits the binding of mouse APRIL to BCMA and TACI. Works in vitro and in vivo.
-
-
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13) (Internal Region) antibody
TNFSF13 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13) (AA 105-250) antibodyTNFSF13 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 2C2C11 unconjugated
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13) (AA 105-247) antibodyTNFSF13 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13) (AA 1-247) antibodyTNFSF13 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal G3 unconjugated
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13) (Internal Region) antibodyTNFSF13 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13) (AA 105-250) antibodyTNFSF13 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13) (Center) antibodyTNFSF13 适用: 人, 小鼠, 大鼠, 犬, Cow, Pig WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13) (AA 151-200) antibodyTNFSF13 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13) antibodyTNFSF13 适用: 人 WB, IP, FACS, IF, IHC (p) 宿主: 小鼠 Monoclonal Aprily-8 unconjugated
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13) (AA 105-250) antibodyTNFSF13 适用: Cow FACS 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 1 mg/mL
-
缓冲液
- In PBS.
-
注意事项
- After opening, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles.
-
储存条件
- 4 °C,-20 °C
-
储存方法
-
Stable for at least 1 month after receipt when stored at +4°C.
Stable for at least 1 year after receipt when stored at -20°C.
-
-
- TNFSF13 (Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13))
-
别名
- APRIL
-
UniProt
- Q9D777
-
途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
抗原
-
