TUBB 抗体
Quick Overview for TUBB 抗体 (ABIN7566465)
抗原
See all TUBB 抗体适用
宿主
克隆类型
标记
应用范围
-
-
原理
- anti-beta-Tubulin (beta-monoE), pAb (IN115)
-
产品特性
-
Polyclonal Antibody. Recognizes the posttranslational modification glutamylation with specific reactivity for the GE(*-E)F motive. Because the sequence GEF is specific to several beta-tubulin isotypes, the antibody selectively detects mono-glutamylated beta-tubulin. Applications: ICC, IHC, IP, WB. Source: Rabbit. Polyglutamylation is an evolutionarily conserved protein post-translational modification in which glutamate side chains of variable lengths are formed on the modified protein. The most prominent substrate is tubulin, the building block of microtubules (MTs). Polyglutamylation has been found on both, alpha- and beta-tubulin. It is catalyzed by multiple enzymes that belong to the family of tubulin tyrosine-ligase like (TTLL) enzymes. These TTLLs have substrate (alpha- vs beta-tubulin) as well as reaction (initiation vs elongation) specificities. These subtle changes in levels of polyglutamylation on specific tubulin isoforms could in turn be influencing the diverse MT functions that are regulated by polyglutamylation. The beta-monoE antibody selectively labels glutamylation of beta-tubulin due to its specificity to a sequence motif (GE(*-E)F) that only exists in beta-tubulin isotypes. It allows to specifically detect beta-tubulin glutamylation in brain tubulin, where other antibodies predominantly detect alpha-tubulin glutamylation.
Polyglutamylation is an evolutionarily conserved protein post-translational modification in which glutamate side chains of variable lengths are formed on the modified protein. The most prominent substrate is tubulin, the building block of microtubules (MTs). Polyglutamylation has been found on both, alpha- and beta-tubulin. It is catalyzed by multiple enzymes that belong to the family of tubulin tyrosine-ligase like (TTLL) enzymes. These TTLLs have substrate (alpha- vs beta-tubulin) as well as reaction (initiation vs elongation) specificities. These subtle changes in levels of polyglutamylation on specific tubulin isoforms could in turn be influencing the diverse MT functions that are regulated by polyglutamylation. The beta-monoE antibody selectively labels glutamylation of beta-tubulin due to its specificity to a sequence motif (GE(*-E)F) that only exists in beta-tubulin isotypes. It allows to specifically detect beta-tubulin glutamylation in brain tubulin, where other antibodies predominantly detect alpha-tubulin glutamylation.
-
纯化方法
- Puified
-
纯度
- >95 % (SDS-PAGE)
-
免疫原
- Synthetic peptide corresponding to D431EQGEFE(E-COOH*)EEEG441-NH2 of human tubulin beta-2A chain (*secondary glutamate branched from gamma-carboxyl group of glutamate as isopeptide bond).
-
-
anti-Tubulin, beta (TUBB) antibody
TUBB 适用: 人, 小鼠, 大鼠, Pig, 小鸡, Tetrahymena, 鱼, Nicotiana tabacum, Arabidopsis, Paramecium WB, ICC, IHC (p) 宿主: 小鼠 Monoclonal TU-06 unconjugated
anti-Tubulin, beta (TUBB) antibodyTUBB 适用: 人 WB, IF, ICC, IHC (p), IHC (wm) 宿主: 兔 Polyclonal unconjugated
anti-Tubulin, beta (TUBB) (AA 61-160) antibodyTUBB 适用: 人, 小鼠, 大鼠, 兔 WB, ELISA, FACS, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Tubulin, beta (TUBB) (AA 170-419) antibodyTUBB 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Tubulin, beta (TUBB) (AA 170-419) antibodyTUBB 适用: 人 WB 宿主: 小鼠 Monoclonal C3 unconjugated
anti-Tubulin, beta (TUBB) (AA 170-419) antibodyTUBB 适用: 人 WB, IHC, ICC, IP 宿主: 小鼠 Monoclonal C6 unconjugated
anti-Tubulin, beta (TUBB) (AA 1-444) antibodyTUBB 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 3B3 unconjugated
anti-Tubulin, beta (TUBB) (AA 160-447) antibodyTUBB 适用: 人, 小鼠 WB, IHC, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Tubulin, beta (TUBB) antibodyTUBB 适用: 人, 小鼠, 大鼠 WB, IF, IHC (p) 宿主: 小鼠 Monoclonal 87CT59-3-7 unconjugated
anti-Tubulin, beta (TUBB) (AA 1-50) antibodyTUBB 适用: 人, 小鼠, 大鼠, Pig WB, ELISA, FACS, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 1 mg/mL
-
缓冲液
- In PBS containing 0.02 % Proclin 300.
-
注意事项
- After opening, prepare aliquots and store at -20 °C.Avoid freeze/thaw cycles.
-
储存条件
- 4 °C,-20 °C
-
储存方法
-
+4°C
Stable for at least 1 year after receipt when stored at -20°C.
-
-
- TUBB (Tubulin, beta (TUBB))
-
别名
- beta-Tubulin
-
UniProt
- Q13885
-
途径
- Microtubule Dynamics, M Phase
抗原
-
