Recombinant SARS-CoV-2 Nucleocapsid 抗体
Quick Overview for Recombinant SARS-CoV-2 Nucleocapsid 抗体 (ABIN7566446)
抗原
See all SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- anti-SARS-CoV-2 N Protein, mAb (rec.) (Capsi-1) (preservative free)
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产品特性
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Recombinant Antibody. Recognizes and binds to recombinant SARS-CoV-2 N protein. Application: ELISA. Liquid, in PBS ( pH 7.4). Coronaviruses (CoVs) are enveloped non-segmented positive-sense single-stranded RNA viruses and can infect respiratory, gastrointestinal, hepatic and central nervous system of human and many other wild animals. Recently, a new severe acute respiratory syndrome beta-coronavirus called SARS-CoV-2 (or 2019-nCoV) has emerged, which causes an epidemic of acute respiratory syndrome (called coronavirus human disease 2019 or COVID-19). SARS-CoV-2 shares 79.5 % sequence identity with SARS-CoV and is 96.2 % identical at the genome level to the bat coronavirus BatCoV RaTG133, suggesting it had originated in bats. SARS-CoV-2 contains 4 structural proteins, including Envelope (E), Membrane (M), Nucleocapsid (N) and Spike (S), which is a transmembrane protein, composed of two subunits S1 and S2. The S1 subunit contains a receptor binding domain (RBD), which binds to the cell surface receptor Angiotensin-Converting Enzyme 2 (ACE2) present at the surface of epithelial cells, causing mainly infection of human respiratory cells. The N protein contains two domains, both of them bind the virus RNA genome via different mechanisms.
Coronaviruses (CoVs) are enveloped non-segmented positive-sense single-stranded RNA viruses and can infect respiratory, gastrointestinal, hepatic and central nervous system of human and many other wild animals. Recently, a new severe acute respiratory syndrome beta-coronavirus called SARS-CoV-2 (or 2019-nCoV) has emerged, which causes an epidemic of acute respiratory syndrome (called coronavirus human disease 2019 or COVID-19). SARS-CoV-2 shares 79.5 % sequence identity with SARS-CoV and is 96.2 % identical at the genome level to the bat coronavirus BatCoV RaTG133, suggesting it had originated in bats. SARS-CoV-2 contains 4 structural proteins, including Envelope (E), Membrane (M), Nucleocapsid (N) and Spike (S), which is a transmembrane protein, composed of two subunits S1 and S2. The S1 subunit contains a receptor binding domain (RBD), which binds to the cell surface receptor Angiotensin-Converting Enzyme 2 (ACE2) present at the surface of epithelial cells, causing mainly infection of human respiratory cells. The N protein contains two domains, both of them bind the virus RNA genome via different mechanisms.
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纯化方法
- Puified
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纯度
- >95 % (SDS-PAGE)
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内毒素水平
- <1EU/mg (LAL method)
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免疫原
- Recombinant SARS-CoV-2 nucleocapsid protein.
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亚型
- IgG1
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Liquid, in PBS ( pH 7.4).
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储存液
- Without preservative
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注意事项
- Avoid freeze/thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
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+4°C
Stable for at least 1 year after receipt when stored at -20°C.
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- SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)
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别名
- SARS-CoV-2 N Protein
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物质类
- Viral Protein
抗原
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