GSDMD 抗体
Quick Overview for GSDMD 抗体 (ABIN7566424)
抗原
See all GSDMD 抗体适用
宿主
克隆类型
标记
应用范围
-
-
原理
- anti-Gasdermin D (mouse), pAb (IN110)
-
产品特性
-
Polyclonal Antibody. Recognizes full-length and cleaved C-terminus domain of mouse gasdermin D. Does not cross-react with human gasdermin D. Source/Host: Guinea pig. Applications: ELISA, WB. Liquid. In PBS containing 10 % glycerol and 0.02 % sodium azide. Inflammasomes are multimeric protein complexes that comprise a sensor (e.g. NLRP3), an adaptor (ASC/Pycard) and the procaspase-1. An inflammasome assembles in response to a diverse range of pathogen-associated or danger-associated molecular patterns (PAMPs or DAMPs). The inflammasome platform leads to activation of caspase-1, which further induces maturation of interleukin-1beta and -18 (IL-1beta and IL-18) through proteolytic cleavage of pro-IL-1beta and pro-IL-18. Activated caspase-1, and also the recently characterized caspase-11 non-canonical inflammasome pathway, also cleave the intracellular gasdermin D, which leads to a particular form of inflammatory cell death called pyroptosis. The gasdermin family members contain N-terminal domains that are capable of forming membrane pores to induce cytolysis, whereas the C-terminal domains of gasdermins function as inhibitors of such cytolysis through intramolecular domain association. Caspase-1 or -11 cleavage of gasdermin D is required for regulation of pyroptosis: upon protease cleavage of the gasdermin N- and C-domain linker, the disruption of the intramolecular domain interaction in the presence of lipids releases the N-domain to assemble oligomeric membrane pores that trigger cell death. Gasdermin D seems to be a key effector in the LPS-induced lethal sepsis.
Inflammasomes are multimeric protein complexes that comprise a sensor (e.g. NLRP3), an adaptor (ASC/Pycard) and the procaspase-1. An inflammasome assembles in response to a diverse range of pathogen-associated or danger-associated molecular patterns (PAMPs or DAMPs). The inflammasome platform leads to activation of caspase-1, which further induces maturation of interleukin-1beta and -18 (IL-1beta and IL-18) through proteolytic cleavage of pro-IL-1beta and pro-IL-18. Activated caspase-1, and also the recently characterized caspase-11 non-canonical inflammasome pathway, also cleave the intracellular gasdermin D, which leads to a particular form of inflammatory cell death called pyroptosis. The gasdermin family members contain N-terminal domains that are capable of forming membrane pores to induce cytolysis, whereas the C-terminal domains of gasdermins function as inhibitors of such cytolysis through intramolecular domain association. Caspase-1 or -11 cleavage of gasdermin D is required for regulation of pyroptosis: upon protease cleavage of the gasdermin N- and C-domain linker, the disruption of the intramolecular domain interaction in the presence of lipids releases the N-domain to assemble oligomeric membrane pores that trigger cell death. Gasdermin D seems to be a key effector in the LPS-induced lethal sepsis.
-
纯化方法
- Puified
-
纯度
- >95 % (SDS-PAGE)
-
免疫原
- Recombinant mouse gasdermin D (C-terminus).
-
-
anti-Gasdermin D (GSDMD) (AA 1-484) antibody
GSDMD 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 3F12-1B2 unconjugated
anti-Gasdermin D (GSDMD) (AA 276-484) antibodyGSDMD 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Gasdermin D (GSDMD) (AA 1-485) antibodyGSDMD 适用: 人 WB, ELISA, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 3F12-1B2 unconjugated
anti-Gasdermin D (GSDMD) antibodyGSDMD 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Gasdermin D (GSDMD) antibodyGSDMD 适用: 人 WB, IF 宿主: 兔 Monoclonal unconjugated
anti-Gasdermin D (GSDMD) (AA 1-326) antibodyGSDMD 适用: 大鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Gasdermin D (GSDMD) (AA 1-256) antibodyGSDMD 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Gasdermin D (GSDMD) (AA 232-484) antibodyGSDMD 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Gasdermin D (GSDMD) (AA 1-484) antibodyGSDMD 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Gasdermin D (GSDMD) (AA 1-484) antibodyGSDMD 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 1 mg/mL
-
缓冲液
- In PBS containing 10 % glycerol and 0.02 % sodium azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- After opening, prepare aliquots and store at -20 °C.Avoid freeze/thaw cycles.
-
储存条件
- 4 °C,-20 °C
-
储存方法
-
+4°C
Stable for at least 1 year after receipt when stored at -20°C.
-
-
- GSDMD (Gasdermin D (GSDMD))
-
别名
- Gasdermin D
-
UniProt
- Q9D8T2
-
途径
- Inflammasome
抗原
-
