GSDMD 抗体
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Quick Overview for GSDMD 抗体 (ABIN7566424)
抗原
See all GSDMD 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- anti-Gasdermin D (mouse), pAb (IN110)
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产品特性
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Polyclonal Antibody. Recognizes full-length and cleaved C-terminus domain of mouse gasdermin D. Does not cross-react with human gasdermin D. Source/Host: Guinea pig. Applications: ELISA, WB. Liquid. In PBS containing 10 % glycerol and 0.02 % sodium azide. Inflammasomes are multimeric protein complexes that comprise a sensor (e.g. NLRP3), an adaptor (ASC/Pycard) and the procaspase-1. An inflammasome assembles in response to a diverse range of pathogen-associated or danger-associated molecular patterns (PAMPs or DAMPs). The inflammasome platform leads to activation of caspase-1, which further induces maturation of interleukin-1beta and -18 (IL-1beta and IL-18) through proteolytic cleavage of pro-IL-1beta and pro-IL-18. Activated caspase-1, and also the recently characterized caspase-11 non-canonical inflammasome pathway, also cleave the intracellular gasdermin D, which leads to a particular form of inflammatory cell death called pyroptosis. The gasdermin family members contain N-terminal domains that are capable of forming membrane pores to induce cytolysis, whereas the C-terminal domains of gasdermins function as inhibitors of such cytolysis through intramolecular domain association. Caspase-1 or -11 cleavage of gasdermin D is required for regulation of pyroptosis: upon protease cleavage of the gasdermin N- and C-domain linker, the disruption of the intramolecular domain interaction in the presence of lipids releases the N-domain to assemble oligomeric membrane pores that trigger cell death. Gasdermin D seems to be a key effector in the LPS-induced lethal sepsis.
Inflammasomes are multimeric protein complexes that comprise a sensor (e.g. NLRP3), an adaptor (ASC/Pycard) and the procaspase-1. An inflammasome assembles in response to a diverse range of pathogen-associated or danger-associated molecular patterns (PAMPs or DAMPs). The inflammasome platform leads to activation of caspase-1, which further induces maturation of interleukin-1beta and -18 (IL-1beta and IL-18) through proteolytic cleavage of pro-IL-1beta and pro-IL-18. Activated caspase-1, and also the recently characterized caspase-11 non-canonical inflammasome pathway, also cleave the intracellular gasdermin D, which leads to a particular form of inflammatory cell death called pyroptosis. The gasdermin family members contain N-terminal domains that are capable of forming membrane pores to induce cytolysis, whereas the C-terminal domains of gasdermins function as inhibitors of such cytolysis through intramolecular domain association. Caspase-1 or -11 cleavage of gasdermin D is required for regulation of pyroptosis: upon protease cleavage of the gasdermin N- and C-domain linker, the disruption of the intramolecular domain interaction in the presence of lipids releases the N-domain to assemble oligomeric membrane pores that trigger cell death. Gasdermin D seems to be a key effector in the LPS-induced lethal sepsis.
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纯化方法
- Puified
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纯度
- >95 % (SDS-PAGE)
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免疫原
- Recombinant mouse gasdermin D (C-terminus).
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anti-Gasdermin D (GSDMD) antibody
GSDMD 适用: 人 WB, IF, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-Gasdermin D (GSDMD) (AA 1-484) antibodyGSDMD 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 3F12-1B2 unconjugated
anti-Gasdermin D (GSDMD) (AA 276-484) antibodyGSDMD 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Gasdermin D (GSDMD) antibodyKO Validated GSDMD 适用: 人 WB, IF (p) 宿主: 兔 Monoclonal 25GB6170 unconjugated Recombinant Antibody
anti-Gasdermin D (GSDMD) antibodyGSDMD 适用: 人 WB, IF 宿主: 兔 Monoclonal unconjugated
anti-Gasdermin D (GSDMD) antibodyGSDMD 适用: 人 WB, IF, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Gasdermin D (GSDMD) (AA 1-485) antibodyGSDMD 适用: 人 WB, ELISA, IF, IHC, IHC (p) 宿主: 小鼠 Monoclonal 3F12-1B2 unconjugated
anti-Gasdermin D (GSDMD) (AA 1-326) antibodyGSDMD 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Gasdermin D (GSDMD) (AA 1-256) antibodyGSDMD 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Gasdermin D (GSDMD) (AA 232-484) antibodyGSDMD 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- In PBS containing 10 % glycerol and 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- After opening, prepare aliquots and store at -20 °C.Avoid freeze/thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
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+4°C
Stable for at least 1 year after receipt when stored at -20°C.
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- GSDMD (Gasdermin D (GSDMD))
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别名
- Gasdermin D
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UniProt
- Q9D8T2
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途径
- Inflammasome
抗原
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