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XDH 抗体 (HRP)

This anti-XDH antibody is a 兔 多克隆 antibody detecting XDH in WB, IP 和 DB. Suitable for Cow.
产品编号 ABIN7565995
发货至: 中国

Quick Overview for XDH 抗体 (HRP) (ABIN7565995)

抗原

See all XDH 抗体
XDH (Xanthine Dehydrogenase (XDH))

适用

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Cow

宿主

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克隆类型

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多克隆

标记

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This XDH antibody is conjugated to HRP

应用范围

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Western Blotting (WB), Immunoprecipitation (IP), Dot Blot (DB)
  • 原理

    Xanthine Oxidase Antibody Peroxidase Conjugated

    交叉反应 (详细)

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Xanthine Oxidase [Bovine Buttermilk].

    产品特性

    Synonyms: rabbit anti-Xanthine Oxidase Antibody Peroxidase Conjugation, HRP Conjugated rabbit anti-Xanthine Oxidase Antibody, Xanthine dehydrogenase antibody, Xanthine dehydrogenase/oxidase antibody, Xanthine oxidase antibody, Xanthine oxidoreductase antibody, xdh antibody, xdha antibody, XO antibody, xor antibody

    纯化方法

    Xanthine Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

    过滤

    Sterile filtered

    免疫原

    Immunogen: Xanthine Oxidase [Bovine Buttermilk]

    Immunogen Type: Native Protein

  • 应用备注

    Application Note: Anti-Xanthine Oxidase Peroxidase conjugated Antibody has been tested by dot blot and is suitable to be assayed against 1.0 μg of Xanthine Oxidase [Bovine Buttermilk] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:35,000 to 1:175,000 of the reconstitution concentration is suggested for this product. Western Blot Dilution: 1:250 Immunoprecipitation Dilution: 1:100 Other: User Optimized

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1.0 mg/mL

    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

    有效期

    12 months
  • 抗原

    XDH (Xanthine Dehydrogenase (XDH))

    别名

    XDH

    背景

    Background: Xanthine Oxidase is a key enzyme in purine degradation. It catalyzes the oxidation of hypoxanthine to xanthine, xanthine to uric acid, and contributes to the generation of reactive oxygen species.

    基因ID

    280960

    UniProt

    P80457

    途径

    Positive Regulation of Endopeptidase Activity
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