TRBOTR 抗体 (HRP)
This 兔 多克隆 antibody specifically detects TRBOTR in DB, ELISA, IHC 和 WB. It exhibits reactivity toward Cow.
产品编号 ABIN7565992
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
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Beijing Economic Technological Development Area
Room 801-803
Quick Overview for TRBOTR 抗体 (HRP) (ABIN7565992)
抗原
TRBOTR
适用
Cow
宿主
兔
克隆类型
多克隆
标记
HRP
应用范围
Dot Blot (DB), ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
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原理
- Trypsin Antibody Peroxidase Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Trypsin [Bovine Pancreas].
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产品特性
- Synonyms: rabbit anti-trypsin antibody HRP conjugation, peroxidase conjugated rabbit anti-trypsin antibody, Anionic trypsinogen antibody, Beta trypsin antibody, Brain trypsinogen antibody, Cationic Trypsinogen antibody, Digestive Zymogen antibody, Mesotrypsin antibody, Mesotrypsinogen antibody
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纯化方法
- Trypsin Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Anti-Trypsin Antibody was produced by repeated immunizations with bovine pancreatic trypsin protein purified.
Immunogen Type: Native Protein
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应用备注
- Application Note: Anti-Trypsin Peroxidase Antibody has been tested by ELISA and dot blot and is suitable for western blotting. Researchers should determine optimal titers for applications that are not stated below. Immunohistochemistry Dilution: User Optimized Western Blot Dilution: 1:100 - 1:500 Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- TRBOTR
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背景
- Background: Anti-Trypsin Antibody detects trypsin. Trypsin is a serine protease found in the digestive system of many vertebrates, where it hydrolyses proteins. It belongs to the peptidase S1 family. Trypsin is produced in the pancreas as the inactive proenzyme trypsinogen. It cleaves peptides on the C-terminal side of lysine and arginine amino acid residues. Trypsin hydrolyzes ester and amide linkages of several synthetic substrates. Anti-Trypsin Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction and Biochemistry research.
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基因ID
- 780933
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UniProt
- P00760
抗原
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