Pol 抗体
The 小鸡 多克隆 anti-Pol antibody is suitable to detect Pol in samples from 小鼠. It has been validated for ELISA.
产品编号 ABIN7565984
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
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Beijing Economic Technological Development Area
Room 801-803
Quick Overview for Pol 抗体 (ABIN7565984)
抗原
Pol
适用
小鼠
宿主
小鸡
克隆类型
多克隆
标记
非结合性
应用范围
ELISA
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原理
- L1/ORF2 Antibody
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交叉反应 （详细）
- Anti-L1/ORF2 Antibody is directed against three regions within the endonuclease domain of L1 ORF2 protein.
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产品特性
- Synonyms: chicken anti-L1/ORF2 Antibody, chicken anti-L1-ORF2 Antibody, L1 (LINE-1), Long INterspersed Element , retrotransposon ORF2
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纯化方法
- This product is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above.
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过滤
- Sterile filtered
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免疫原
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Immunogen: This IgY fraction antibody was prepared from eggs of chickens laid after repeated immunizations with three synthetic peptides conjugated to keyhole limpet hemocyanin (KLH). The peptides correspond to regions within the endonuclease domain of L1/ORF2 protein.
Immunogen Type: Conjugated Peptide
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应用备注
- Application Note: L1/ORF2 Antibody has been tested for use in ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 149.5 kDa in size corresponding to L1 protein by western blotting in the appropriate cell lysate or extract. ELISA Dilution: 1:20,000-1:100,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.21 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- Pol
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物质类
- Viral Protein
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背景
- Background: L1/ORF2 is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. The L1 (LINE-1), or Long INterspersed Element , retrotransposon ORF2 is the most common open reading frame in the human genome, present in various forms in many thousands of copies. This large family of proteins includes magnesium dependent endonucleases and a large number of phosphatases involved in intracellular signaling. Both intact ORF1 and ORF2 are absolutely required for autonomous retro-transposition. ORF2 encodes an endonuclease, reverse transcriptase, and zinc knuckle domains. The expression of ORF2 appears to be tightly regulated except in germ line tissues, embryonic tissues, and certain cancers including teratomas, testicular cancers, and leukemias. This antibody is intended for use in studying control of L1 retrotransposons.
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UniProt
- P11369
抗原
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