Protein A 抗体
Quick Overview for Protein A 抗体 (ABIN7565957)
抗原
See all Protein A 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Protein A Antibody
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Protein A [Staphylococcus aureus] and anti-Chicken Serum.
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产品特性
- Synonyms: chicken anti-Protein A Antibody, chicken anti Protein A, chicken anti PA
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纯化方法
- This Anti-Protein A Antibody is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above.
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过滤
- Sterile filtered
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免疫原
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Immunogen: This IgY fraction antibody was prepared from eggs of chickens laid after repeated immunizations with Protein A [Staphylococcus aureus].
Immunogen Type: Native Protein
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anti-Protein A antibody
适用: Staphylococcus aureus ELISA, WB, IF (p), IF (cc), IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Protein A antibody (Rhodamine)适用: Staphylococcus aureus IF 宿主: 山羊 Polyclonal Rhodamine
anti-Protein A antibody适用: Staphylococcus aureus ELISA, WB, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Protein A antibody适用: Staphylococcus aureus ELISA, WB, IP 宿主: 山羊 Polyclonal unconjugated
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应用备注
- Application Note: Anti-PROTEIN A (CHICKEN) Antibody has been tested by western blotting and suitable for ELISA. Although not tested, this antibody may also be useful for neutralization assays, immunohistochemistry, flow Cytometry, immunoprecipitation, immunodiffusion, conjugation and most immunological methods requiring high titer and specificity. Western Blot Dilution: 1:500 - 1:2,000 ELISA Dilution: 1:500 - 1:2,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- Protein A
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背景
- Background: Protein A is 56 kDA surface protein found in the cell wall of Staphylococcus aureus. Protein A has a high affinity to Human IgG, Mouse IgG and others and is therefore suitable for purification and detection of immunoglobulins.
抗原
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