EFNB1/EFNB2/EFNB3 (C-Term) 抗体
This 兔 多克隆 antibody specifically detects EFNB1/EFNB2/EFNB3 in ELISA, FM, IF 和 WB. It exhibits reactivity toward 人.
产品编号 ABIN7565950
发货至:
中国
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Quick Overview for EFNB1/EFNB2/EFNB3 (C-Term) 抗体 (ABIN7565950)
抗原
EFNB1/EFNB2/EFNB3
适用
人
宿主
兔
克隆类型
多克隆
标记
非结合性
应用范围
ELISA, Fluorescence Microscopy (FM), Immunofluorescence (IF), Western Blotting (WB)
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抗原表位
- C-Term
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原理
- Ephrin B1, B2, B3 Antibody
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交叉反应 （详细）
- This affinity purified antibody is directed against human Ephrin B1, B2, B3.
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产品特性
- Synonyms: Rabbit Anti-Ephrin B1, B2, B3 Antibody, Ephrin-B1, Ephrin-B2, Ephrin-B3, ELK Ligand, EPLG2, LERK2, EFL3, Craniofrontonasal Syndrome (Craniofrontonasal Dysplasia), EPH-Related Receptor Transmembrane Ligand ELK-L3, Eph-Related Receptor Tyrosine Kinase Ligand 2, Eph-Related Receptor Tyrosine Kinase Ligand 8, EPH-Related Receptor Tyrosine Kinase Ligand 2, LERK-2, LERK-8, Elk-L, EFL-3, ELK-L, CFND, EFL6, EFB1, CFNS
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纯化方法
- This affinity purified antibody is directed against human Ephrin B1, B2, B3.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Anti-Ephrin B1, B2, B3 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to C-terminal portion of human Ephrin B1, B2, B3 conjugated to Keyhole Limpet Hemocyanin (KLH).
Immunogen Type: Conjugated Peptide
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应用备注
- Application Note: Anti-Ephrin B1, B2, B3 Antibody has been tested in ELISA, Western Blot, and Immunofluorescence. Expect a band at ~35-38 kDa in western blot using appropriate lysates or tissues. Positive control used Xenopus laevis and mouse kidney in WB and Xenopus laevis in IF. Western Blot Dilution: 1:1000 ELISA Dilution: 1:10,000-50,000 IF Microscopy Dilution: 1:5000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.05 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- EFNB1/EFNB2/EFNB3
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别名
- EFNB1, Efnb2, EFNB3
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背景
- Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) (NCI). Ephrin B1, B2, B3 are cell surface transmembrane ligands for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binding to Eph receptors residing on adjacent cells leads to contact-dependent bidirectional signaling into neighboring. Ephrin B1 shows high affinity for the receptor tyrosine kinase EPHB1/ELK and can bind EPHB2 and EPHB3. Ephrin B3 may play a pivotal role in forebrain function. They binds to, and induces collapse of, commissural axons/growth cones in vitro. And may play a role in constraining the orientation of longitudinally projecting axons. Anti-Ephrin B1, B2, B3 Antibody is useful for researchers interested in Developmental Biology and Tyrosine Kinases/Adaptors.
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基因ID
- 1947
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NCBI登录号
- NP_004420
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UniProt
- P52800
抗原
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