SETDB2 抗体
Quick Overview for SETDB2 抗体 (ABIN7565943)
抗原
See all SETDB2 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Setdb2 Antibody
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交叉反应 （详细）
- This Protein A purified antibody is directed against mouse Setdb2.
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产品特性
- Synonyms: rabbit anti-Setdb2 Antibody, Histone-lysine N-methyltransferase SETDB2, SET domain bifurcated 2, Setdb 2 antibody, Gm293
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纯化方法
- The product was purified from monospecific antiserum by Protein A affinity chromatography.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Setdb2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a full length protein corresponding to mouse Setdb2.
Immunogen Type: Recombinant Protein
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anti-SET Domain, Bifurcated 2 (SETDB2) (N-Term) antibody
Verified SETDB2 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-SET Domain, Bifurcated 2 (SETDB2) (AA 451-550) antibodySETDB2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2F4 unconjugated
anti-SET Domain, Bifurcated 2 (SETDB2) (AA 620-719) antibodySETDB2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1E2 unconjugated
anti-SET Domain, Bifurcated 2 (SETDB2) (N-Term) antibodySETDB2 适用: 人, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-SET Domain, Bifurcated 2 (SETDB2) (AA 451-550) antibodySETDB2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2D11 unconjugated
anti-SET Domain, Bifurcated 2 (SETDB2) (AA 71-120) antibodySETDB2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-SET Domain, Bifurcated 2 (SETDB2) (AA 1-280) antibodySETDB2 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
- Application Note: Anti-Setdb2 antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 80.6 kDa in size corresponding to Setdb2 by western blotting in the appropriate cell lysate or extract. Western Blot Dilution: 1:10,000 - 1:100,000 ELISA Dilution: 1:2,500 - 1:10,000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.98 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- SETDB2 (SET Domain, Bifurcated 2 (SETDB2))
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别名
- Setdb2
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背景
- Background: Setdb2 is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) (NCI). Setdb2 and the neighboring gene Phf11 were linked to familial atopy/ asthma and are in a "critical region" commonly deleted in CLL. Setdb2 is a candidate histone methyltransferase as a member of the Su(var) 3-9 family of SET domain-containing enzymes. Other predicted domains in Setdb2 include a methylcytosine binding domain and a large bifurcation domain interrupting the putative histone methyltransferase (SET) domain whose function is unknown. Anti-Setdb2 antibody is ideal for researchers interested in Cancer Research and Epigenetics Research.
抗原
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