HIST1H3A 抗体 (C-Term)
Quick Overview for HIST1H3A 抗体 (C-Term) (ABIN7565940)
抗原
See all HIST1H3A 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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原理
- Histone H3 Antibody
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交叉反应 （详细）
- Anti-Histone H3 is directed against the human histone 3 protein.
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产品特性
- Synonyms: rabbit anti-histone H3 antibody, Histone H3.1, H3F, H3FA, H3FB, H3FL, H3FL, H3FC, H3FD, H3FI, H3FH, H3FK, H3FF, H3FJ, Histone 3 Antibody
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纯化方法
- The product was protein A purified from monospecific antiserum by immunoaffinity chromatography using protein A coupled to agarose beads.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Anti-Histone-3 was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to the c-terminus region of human histone-3.
Immunogen Type: Conjugated Peptide
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anti-Histone Cluster 1, H3a (HIST1H3A) (acLys27) antibody
HIST1H3A 适用: 人 ELISA, WB, IF, ICC, ChIP, IP 宿主: 兔 Polyclonal unconjugated
anti-Histone Cluster 1, H3a (HIST1H3A) antibodyHIST1H3A 适用: 人 ELISA, WB, IHC, IF, FACS 宿主: 兔 Monoclonal 27F2 unconjugated Recombinant Antibody
anti-Histone Cluster 1, H3a (HIST1H3A) (acLys4) antibodyHIST1H3A 适用: 人 ELISA, WB, IF, ChIP, IP 宿主: 兔 Polyclonal unconjugated
anti-Histone Cluster 1, H3a (HIST1H3A) (H3K4me2) antibodyHIST1H3A 适用: 人 ELISA, WB, IF, ICC, FACS 宿主: 兔 Monoclonal 6F6 unconjugated Recombinant Antibody
anti-Histone Cluster 1, H3a (HIST1H3A) (pSer10) antibodyHIST1H3A 适用: 人 ELISA, WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Histone Cluster 1, H3a (HIST1H3A) (H3K27me2) antibodyHIST1H3A 适用: 人 ELISA, WB, IF, ChIP, DB, FM 宿主: 兔 Polyclonal unconjugated
anti-Histone Cluster 1, H3a (HIST1H3A) (acLys9) antibodyHIST1H3A 适用: 人 ELISA, WB, ICC, ChIP 宿主: 兔 Polyclonal unconjugated
anti-Histone Cluster 1, H3a (HIST1H3A) (acLys37) antibodyHIST1H3A 适用: 人 ELISA, WB, IF, ChIP 宿主: 兔 Polyclonal unconjugated
anti-Histone Cluster 1, H3a (HIST1H3A) (acLys36) antibodyHIST1H3A 适用: 人 ELISA, WB, IF, ChIP 宿主: 兔 Polyclonal unconjugated
anti-Histone Cluster 1, H3a (HIST1H3A) (acLys23) antibodyHIST1H3A 适用: 人 ELISA, WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Application Note: Histone H3 antibody has been tested for use in ELISA and western blot. This antibody is suitable for IHC. For western blots expect a band of approximately 15.4 kDa in size corresponding to the Histone 3 protein. Specific conditions for reactivity should be optimized by the end user. Immunohistochemistry Dilution: User Optimized Western Blot Dilution: 1.0 μg/mL ELISA Dilution: 5.0 μg/mL Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.98 mg/mL
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缓冲液
- Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Preservative: 0.01 % (w/v) Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store H3 antibody at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- HIST1H3A (Histone Cluster 1, H3a (HIST1H3A))
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别名
- HIST1H3A
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背景
- Background: Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Histone proteins are highly post-translationally modified with Histone H3 being the most extensively modified of the five histones. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone H3 Antibody is ideal for investigators involved in Cell Signaling, Epigenetics, Nuclear Signaling research and Signal Transduction research.
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基因ID
- 8350
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NCBI登录号
- NP_003520
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UniProt
- P68431
抗原
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