Streptavidin 抗体 (HRP)
Quick Overview for Streptavidin 抗体 (HRP) (ABIN7565912)
抗原
See all Streptavidin 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Streptavidin Antibody Peroxidase Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Streptavidin [Streptomyces avidinii].
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产品特性
- Synonyms: rabbit anti-Streptavidin Antibody HRP Conjugation, Peroxidase conjugated rabbit anti-Streptavidin antibody, Anti-Streptavidin HRP Antibody
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纯化方法
- Streptavidin Antibody Peroxidase Conjugate is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Streptavidin [Streptomyces avidinii]
Immunogen Type: Native Protein
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anti-Streptavidin (AA 25-183) antibody
适用: Streptomyces avidinii ELISA, WB 宿主: 小鼠 Monoclonal 1C2 unconjugated
anti-Streptavidin (C-Term) antibody适用: Streptomyces avidinii WB 宿主: 山羊 Polyclonal unconjugated
anti-Streptavidin (AA 25-183) antibody适用: Streptomyces avidinii ELISA, WB 宿主: 小鼠 Monoclonal 1C2 unconjugated
anti-Streptavidin antibody (FITC)适用: Streptomyces avidinii FACS, IF 宿主: 兔 Polyclonal FITC
anti-Streptavidin antibody适用: Streptomyces avidinii ELISA, WB, IHC, DB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Application Note: Anti-Streptavidin Peroxidase antibody has been tested by ELISA and western blot. This product has been assayed against 1.0 μg of Streptavidin in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) for 30 minutes at room temperature. Optimal concentrations in immunoassays should be determined by researcher. ELISA Dilution: 1:2,000-1:10,000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- Streptavidin
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背景
- Background: Anti-streptavidin antibody detects Streptavidin, a 60 kDa protein purified from the bacterium Streptomyces avidinii. Streptavidin is a homo-tetrameric protein having a very high affinity for biotin (vitamin B7). Streptavidin bound biotin has a dissociation constant (Kd) on the order of about 10^-14 Mol/L. Streptavidin and biotin are used extensively in molecular biology and bionanotechnology due to the resistance of streptavidin-biotin complex to organic solvents, denaturants (e.g. guanidinium chloride), detergents (e.g. SDS, Triton), proteolytic enzymes, and extremes of temperature and pH .
抗原
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