MBP Tag 抗体 (Biotin)
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北京 101111
Quick Overview for MBP Tag 抗体 (Biotin) (ABIN7565908)
抗原
适用
宿主
克隆类型
标记
应用范围
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原理
- Maltose Binding Protein (MBP) Epitope Tag Antibody Biotin Conjugated
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交叉反应 （详细）
- This IgG purified antibody is directed against MBP and is useful in determining its presence in various assays. This polyclonal anti-MBP tag antibody detects over-expressed proteins containing the MBP epitope tag.
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产品特性
- Synonyms: rabbit anti-MBP Epitope Tag Antibody biotin conjugation, rabbit anti-Maltose Binding Protein Antibody biotin conjugated
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纯化方法
- Purified antibody
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过滤
- Sterile filtered
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免疫原
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Immunogen: This antibody was purified from whole rabbit serum prepared by repeated immunizations with the MBP epitope tag recombinant protein.
Immunogen Type: Recombinant Protein
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anti-MBP Tag antibody
WB 宿主: 兔 Polyclonal RB55937 unconjugated
anti-MBP Tag antibody适用: 标记 WB, ELISA, IP, ICC, IHC, FACS 宿主: 小鼠 Monoclonal 3G1A3 unconjugated
anti-MBP Tag antibodyWB, ELISA, IP 宿主: 兔 Polyclonal unconjugated
anti-MBP Tag antibodyWB 宿主: 小鼠 Monoclonal 8K2 unconjugated
anti-MBP Tag antibodyWB, ELISA 宿主: 小鼠 Monoclonal 5B10B11 unconjugated
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应用备注
- Application Note: Anti-MBP Biotin Conjugated Antibody is optimally suited for monitoring the expression of MBP tagged fusion proteins. As such, anti- MBP/MBP can be used to identify fusion proteins containing the MBP epitope. The antibody recognizes the MBP epitope tag fused to the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against MBP containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry, and other immunodetection techniques. Maltose binding protein is a bacterial protein, which is often used in protein expression studies because it creates a stable fusion product that does not appear to interfere with the bioactivity of the protein of interest. It also allows for its easy purification from bacterial extracts under mild conditions. Anti-MBP is a companion to the pMAL protein expression system and can be used for the detection and purification of MBP-fusion proteins expressed in E. coli. By Western blot, a band is seen at ~ 42 kDa representing MBP. Western Blot Dilution: 1:1,000-1:5,000 ELISA Dilution: 1:10,000-1:50,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- MBP Tag
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物质类
- Tag
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背景
- Background: Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
抗原
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