Pepsin A 抗体 (HRP)
Quick Overview for Pepsin A 抗体 (HRP) (ABIN7565905)
抗原
See all Pepsin A 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Pepsin Antibody Peroxidase Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Pepsin [Porcine Stomach].
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产品特性
- Synonyms: goat anti-Pepsin Antibody HRP Conjugated, Peroxidase Conjugated goat anti-Pepsin Antibody, Pepsin A antibody, Pepsin a precursor antibody, Pepsin antibody, Pepsinogen 1 second locus antibody, Pepsinogen 3 group 1 antibody, Pepsinogen a3 antibody
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纯化方法
- Pepsin is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Pepsin [Porcine Stomach]
Immunogen Type: Native Protein
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anti-Pepsin A antibody
适用: 人, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Pepsin A (Internal Region) antibody适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Pepsin A antibody适用: Pig WB, IHC, ELISA, IP 宿主: 山羊 Polyclonal unconjugated
anti-Pepsin A antibody (Biotin)适用: Pig WB, IHC, ELISA 宿主: 山羊 Polyclonal Biotin
anti-Pepsin A antibody (HRP)适用: Pig WB, IHC, ELISA, DB 宿主: 山羊 Polyclonal HRP
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应用备注
- Application Note: Anti-Pepsin peroxidase has been tested by ELISA, dot blot, and western blot and is useful in immunohistochemistry. Optimal titers should be obtained by researchers if not provided. Immunohistochemistry Dilution: 1:250 - 1:1,250 Western Blot Dilution: 1:500 - 1:2,500 ELISA Dilution: 1:2,000 - 1:10,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- Pepsin A
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别名
- PGA
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背景
- Background: Pepsin is one of three principal protein-degrading, or proteolytic, enzymes in the digestive system, the other two being chymotrypsin and trypsin. Pepsin is commonly used in the preparation produce either an Fab or an F(ab')2 fragment from antibodies.
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基因ID
- 396892
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UniProt
- P00791
抗原
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