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ampC 抗体 (HRP)

ampC 适用: Enterobacter cloacae WB, IP, DB 宿主: 兔 Polyclonal HRP
产品编号 ABIN7565904
发货至: 中国
  • 抗原 See all ampC 抗体
    ampC (beta-Lactamase (ampC))
    适用
    • 5
    • 4
    • 3
    Enterobacter cloacae
    宿主
    • 12
    克隆类型
    • 12
    多克隆
    标记
    • 3
    • 3
    • 3
    • 3
    This ampC antibody is conjugated to HRP
    应用范围
    • 8
    • 4
    • 3
    • 2
    Western Blotting (WB), Immunoprecipitation (IP), Dot Blot (DB)
    原理
    Penicillinase Antibody Peroxidase Conjugated
    交叉反应 (详细)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Penicillinase [Enterobacter cloacae].
    产品特性
    Synonyms: rabbit anti-Penicillinase Antibody Peroxidase Conjugation, HRP Conjugated rabbit anti-Penicillinase Antibody, Aer1 antibody, Beta lactamase 1 antibody, Beta lactamase AER 1 antibody, OXA9 antibody, Oxacillinase antibody, PenPC antibody, Cephalosporinase
    纯化方法
    Penicillinase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
    过滤
    Sterile filtered
    免疫原

    Immunogen: Penicillinase [Enterobacter cloacae]

    Immunogen Type: Native Protein

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  • 应用备注
    Application Note: Anti-Penicillinase Peroxidase conjugated antibody has been tested by dot blot and is suitable to be assayed against 1.0 μg of Penicillinase [Enterobacter cloacae] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product. Western Blot Dilution: 1:250 Immunoprecipitation Dilution: 1:100 Other: User Optimized
    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1.0 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    -20 °C
    储存方法
    Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
    有效期
    12 months
  • 抗原
    ampC (beta-Lactamase (ampC))
    别名
    ampC (ampC 产品)
    背景
    Background: Penicillinase is a specific type of β-lactamase, showing specificity for penicillin, again by hydrolyzing the beta-lactam ring. Molecular weights of the various penicillinases cluster around 50 kDa. Beta-lactamase provides antibiotic resistance by breaking the antibiotics' structure. These antibiotics all have a common element in their molecular structure: a four-atom ring known as a beta-lactam. Through hydrolysis, the lactamase enzyme breaks the β-lactam ring open, deactivating the molecule's antibacterial properties.
    基因ID
    9125732
    UniProt
    P05364
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