Ovalbumin 抗体 (FITC)
Quick Overview for Ovalbumin 抗体 (FITC) (ABIN7565899)
抗原
See all Ovalbumin (OVA) 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Ovalbumin Antibody Fluorescein Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum as well as purified and partially purified Ovalbumin [Hen Egg White].
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产品特性
- Synonyms: rabbit anti-Ovalbumin Antibody FITC Conjugation, fluorescein conjugated rabbit anti-Ovalbumin antibody, Egg albumin antibody, Plakalbumin, Allergen Gal d II, Allergen=Gal d 2, Hen Egg White
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纯化方法
- Ovalbumin Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Ovalbumin [Hen Egg White]
Immunogen Type: Native Protein
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Labeling Ratio
- 3.6
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anti-Ovalbumin (OVA) (AA 1-350) antibody
OVA 适用: 人, 小鼠, 大鼠 WB, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Ovalbumin (OVA) antibodyOVA 适用: 小鸡 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Ovalbumin (OVA) antibody (HRP)OVA 适用: 小鸡 WB, ELISA, IP, IHC 宿主: 兔 Polyclonal HRP
anti-Ovalbumin (OVA) antibodyOVA 适用: 小鸡 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Ovalbumin (OVA) antibodyOVA 适用: Various Species WB 宿主: 小鼠 Monoclonal 3# unconjugated
anti-Ovalbumin (OVA) antibodyOVA 适用: 小鸡 WB, ELISA 宿主: 小鼠 Monoclonal 3G2E1D9 unconjugated
anti-Ovalbumin (OVA) antibodyOVA 适用: Avian WB, ELISA 宿主: 小鼠 Monoclonal 3G2E1D9-3D5F4A8-3G1E1D9 unconjugated
anti-Ovalbumin (OVA) antibody (Biotin)OVA 适用: 小鸡 WB, ELISA, IP, DB 宿主: 兔 Polyclonal Biotin
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应用备注
- Application Note: Anti-Ovalbumin fluorescein conjugated antibody has been tested by ELISA, dot blot, and western blot and is suitable for fluorescent western blot, immunofluorescence microscopy, and flow cytometry or FACS analysis as well as other antibody based fluorescent assays. Researchers should determine optimal titers for applications that are not stated below. Western Blot Dilution: 1:1,000 - 1:5,000 Immunoprecipitation Dilution: 1:100 ELISA Dilution: 1:2,000 - 1:10,000 IF Microscopy Dilution: 1:500 - 1:2,500 Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- Ovalbumin (OVA)
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别名
- SERPINB14
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背景
- Background: Anti-Ovalbumin Antibody detects Ovalbumin. Ovalbumin is the main protein found in egg white, making up 60-65 % of the total protein. It is a non-inhibitory serpin. It is the storage protein of egg white. Anti-Ovalbumin Antibody is ideal for investigators involved in Cell Signaling, Immunology and Signal Transduction research.
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基因ID
- 396058
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UniProt
- P01012
抗原
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