GALM 抗体 (HRP)
Quick Overview for GALM 抗体 (HRP) (ABIN7565898)
抗原
See all GALM 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Mutarotase Antibody Peroxidase Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Mutarotase [Porcine Kidney].
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产品特性
- Synonyms: rabbit anti-Mutarotase Antibody Peroxidase Conjugation, HRP Conjugated rabbit anti-Mutarotase Antibody, Aldose 1 epimerase antibody, BLOCK25 protein antibody, Galactose mutarotase antibody, GALM antibody
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纯化方法
- Mutarotase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Mutarotase [Porcine Kidney]
Immunogen Type: Native Protein
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anti-Galactose Mutarotase (Aldose 1-Epimerase) (GALM) (AA 2-342) antibody
GALM 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Galactose Mutarotase (Aldose 1-Epimerase) (GALM) (C-Term) antibodyGALM 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Galactose Mutarotase (Aldose 1-Epimerase) (GALM) (AA 1-342) antibodyGALM 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Galactose Mutarotase (Aldose 1-Epimerase) (GALM) (AA 251-300) antibodyGALM 适用: 人, 大鼠, 小鼠, 豚鼠, Cow, 马, Hamster, Bat, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Galactose Mutarotase (Aldose 1-Epimerase) (GALM) (AA 36-85) antibodyGALM 适用: 人, 大鼠, 小鼠, Pig, 豚鼠, 犬, 兔, Hamster WB 宿主: 兔 Polyclonal unconjugated
anti-Galactose Mutarotase (Aldose 1-Epimerase) (GALM) (Middle Region) antibodyGALM 适用: 人, 大鼠, 小鼠, 豚鼠, Cow, 犬, 马, 兔, 山羊, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Application Note: Anti-Mutarotase Peroxidase Conjugated Antibody has been tested by ELISA and western blot. This product is assayed against 1.0 μg of Mutarotase [Porcine Kidney] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:1,500 of the reconstitution concentration is suggested for this product. Western Blot Dilution: 1:500 - 1:5,000 Immunoprecipitation Dilution: 1:100 ELISA Dilution: 1:5,000 - 1:20,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- GALM (Galactose Mutarotase (Aldose 1-Epimerase) (GALM))
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别名
- GALM
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背景
- Background: Mutarotase is found especially in mammalian tissues that catalyzes the interconversion of anomeric forms of some sugars: α- and β- forms of d-glucose, l-arabinose, d-galactose, d-xylose, lactose, and maltose.
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基因ID
- 399536
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UniProt
- Q9GKX6
抗原
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