Env (ENV) 抗体
ENV
适用: Mouse Mammary Tumor Virus (MMTV)
ELISA, WB
宿主: 兔
Polyclonal
unconjugated
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- 抗原
- Env (ENV)
- 适用
- Mouse Mammary Tumor Virus (MMTV)
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- 非结合性
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应用范围
- ELISA, Western Blotting (WB)
- 原理
- MMTV Antibody
- 产品特性
- Synonyms: rabbit anti-MMTV Antibody, rabbit anti-mouse mammary tumor virus surface protein antibody, Envelope glycoprotein gp70, Surface protein, Glycoprotein 52, Transmembrane protein, Glycoprotein 36, Mouse mammary tumor virus (strain GR) (MMTV)
- 纯化方法
- Mouse mammary tumor virus surface protein antibody was prepared from monospecific, delipidated and defibrinated antiserum, with addition of sodium azide to 0.01 % and cross adsorbed with 6X HIS.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: MMTV was prepared from whole rabbit serum produced by repeated immunizations with a full length sequence for mouse mammary tumor virus surface protein tagged with His.
Immunogen Type: Native Protein
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- 应用备注
- Application Note: Anti-MMTV Antibody has been tested for use in western blotting. Specific conditions for reactivity should be optimized by the end user. Expect band approximately 41.2 kDa in size corresponding to MMTV surface protein by western blotting in the appropriate cell lysate or extract. Western Blot Dilution: 1:1000-1:2000 ELISA Dilution: 1:10,000-1:20,000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 66 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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- 抗原
- Env (ENV)
- 别名
- env
- 物质类
- Viral Protein
- 背景
- Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) (NCI) and is suitable for Cancer, Enzyme, and Viral research. Mouse mammary tumor virus surface protein binds the virus to the receptor of the host cells triggering transmembrane protein refolding. This activates the fusion peptide and the structure coils and repeats positioning the fusion peptide near the C-terminus region. Inturn increasing close proximity and then fusion of viral and cell membranes. Anti-MMTV is ideal for researchers interested in Cancer, Viral, and Enzyme research.
- UniProt
- P03374
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