DLD 抗体 (HRP)
Quick Overview for DLD 抗体 (HRP) (ABIN7565891)
抗原
See all DLD 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Lipoamide Dehydrogenase Antibody Peroxidase Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Lipoamide Dehydrogenase [Porcine Heart].
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产品特性
- Synonyms: rabbit anti-Lipoamide Dehydrogenase Antibody Peroxidase Conjugation, HRP Conjugated rabbit anti-Lipoamide Dehydrogenase Antibody, Dihydrolipoyl dehydrogenase mitochondrial, Dihydrolipoamide dehydrogenase, Lipoamide Dehydrogenase
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纯化方法
- Lipoamide Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Lipoamide Dehydrogenase [Porcine Heart]
Immunogen Type: Native Protein
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anti-Dihydrolipoamide Dehydrogenase (DLD) (AA 45-271) antibody
DLD 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Dihydrolipoamide Dehydrogenase (DLD) antibodyDLD 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Dihydrolipoamide Dehydrogenase (DLD) antibodyDLD 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-Dihydrolipoamide Dehydrogenase (DLD) antibodyKO Validated DLD 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Dihydrolipoamide Dehydrogenase (DLD) (AA 1-509) antibodyDLD 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 3C1 unconjugated
anti-Dihydrolipoamide Dehydrogenase (DLD) (AA 36-300) antibodyDLD 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Dihydrolipoamide Dehydrogenase (DLD) (AA 238-466) antibodyDLD 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Dihydrolipoamide Dehydrogenase (DLD) antibodyDLD 适用: 人, 小鼠, 大鼠, 犬, 猴 WB, IF, FACS 宿主: 小鼠 Monoclonal 5G7 unconjugated
anti-Dihydrolipoamide Dehydrogenase (DLD) (C-Term) antibodyDLD 适用: 人, 小鼠, 大鼠 WB, ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Dihydrolipoamide Dehydrogenase (DLD) (AA 280-487) antibodyDLD 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Application Note: Anti-Lipoamide Dehydrogenase Peroxidase Conjugated Antibody has been tested by ELISA and Western blot and is assayed against 1.0 μg of Lipoamide Dehydrogenase [Porcine Heart] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:10,000 of the reconstitution concentration is suggested for this product. Western Blot Dilution: 1:500 - 1:5,000 Immunoprecipitation Dilution: 1:100 ELISA Dilution: 1:5,000 - 1:20,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- DLD (Dihydrolipoamide Dehydrogenase (DLD))
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别名
- DLD LAD
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背景
- Background: Lipoamide Dehydrogenase encodes the L protein of the mitochondrial glycine cleavage system. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency.
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基因ID
- 397129, 118675
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UniProt
- P09623
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途径
- Ribonucleoside Biosynthetic Process, Cell RedoxHomeostasis
抗原
-
