GPD1 抗体 (HRP)
Quick Overview for GPD1 抗体 (HRP) (ABIN7565885)
抗原
See all GPD1 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Glycerol-3-Phosphate Dehydrogenase Antibody Peroxidase Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Glycerol-3-Phosphate-Dehydrogenase [Rabbit Muscle].
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产品特性
- Synonyms: goat anti-Glycerol-3-Phosphate Dehydrogenase Antibody HRP Conjugation, Peroxidase Conjugated goat anti-Glycerol-3-Phosphate Dehydrogenase Antibody, FLJ26652 antibody, G3PD antibody, Gdc-1 antibody, Glycerphosphate dehydrogenase antibody, GPD-C antibody, Gpd1 protein antibody
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纯化方法
- Glycerol-3-Phosphate Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Glycerol-3-Phosphate-Dehydrogenase [Rabbit Muscle]
Immunogen Type: Native Protein
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anti-Glycerol-3-Phosphate Dehydrogenase 1 (Soluble) (GPD1) (AA 1-349) antibody
GPD1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3C10-1C4 unconjugated
anti-Glycerol-3-Phosphate Dehydrogenase 1 (Soluble) (GPD1) (AA 1-349) antibodyGPD1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Glycerol-3-Phosphate Dehydrogenase 1 (Soluble) (GPD1) (AA 1-349) antibodyGPD1 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Glycerol-3-Phosphate Dehydrogenase 1 (Soluble) (GPD1) (AA 28-57), (N-Term) antibodyGPD1 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB21027 unconjugated
anti-Glycerol-3-Phosphate Dehydrogenase 1 (Soluble) (GPD1) antibodyGPD1 适用: 人, 小鼠 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Glycerol-3-Phosphate Dehydrogenase 1 (Soluble) (GPD1) (AA 35-65), (N-Term) antibodyGPD1 适用: 人, 小鼠 WB, FACS, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-Glycerol-3-Phosphate Dehydrogenase 1 (Soluble) (GPD1) (AA 1-349) antibodyGPD1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Glycerol-3-Phosphate Dehydrogenase 1 (Soluble) (GPD1) (AA 110-159) antibodyGPD1 适用: 人, 小鼠, 犬, 豚鼠, 马, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Glycerol-3-Phosphate Dehydrogenase 1 (Soluble) (GPD1) (AA 251-300) antibodyGPD1 适用: 人, 斑马鱼, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Glycerol-3-Phosphate Dehydrogenase 1 (Soluble) (GPD1) (N-Term) antibodyGPD1 适用: 人, 小鼠, 兔, 大鼠, 犬, 豚鼠, 马, Cow, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Application Note: Anti-Glycerol-3-Phosphate Dehydrogenase has been tested by western blot and is suitable to be assayed against 1.0 μg of Glycerol-3-Phosphate-Dehydrogenase [Rabbit Muscle] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:2,500 of the reconstitution concentration is suggested for this product. Western Blot Dilution: 1:500 - 1:2,500 ELISA Dilution: 1:1,000 - 1:5,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- GPD1 (Glycerol-3-Phosphate Dehydrogenase 1 (Soluble) (GPD1))
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别名
- GPD1
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背景
- Background: Glycerol-3-phosphate dehydrogenase serves as a major link between carbohydrate metabolism and lipid metabolism. Through the reduction of dihydroxyacetone phosphate into glycerol 3-phosphate, GPDH allows the prompt dephosphorylation of glycerol 3-phosphate into glycerol. It is also a major contributor of electrons to the electron transport chain in the mitochondria. GPDH is responsible for maintaining the redox potential across the inner mitochondrial membrane in glycolysis. Since glycerol is a main subunit in lipid metabolism, its abundance can easily lead to an increase in triglyceride accumulation at a cellular level. As a result, there is a tendency to form adipose tissue leading to an accumulation of fat that favors obesity. GPDH has also been found to play a role in Brugada syndrome. Mutations in the gene encoding GPD1 have been proven to cause defects in the electron transport chain. This conflict with NAD+/NADH levels in the cell is believed to contribute to defects in cardiac sodium ion channel regulation and can lead to a lethal arrythmia during infancy.
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基因ID
- 100339469, 3043365
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UniProt
- P08507
抗原
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