Glycerol Kinase 抗体 (HRP)
Quick Overview for Glycerol Kinase 抗体 (HRP) (ABIN7565884)
抗原
See all Glycerol Kinase (GK) 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Glycerol Kinase Antibody HRP Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Glycerol Kinase [Cellulomonas species].
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产品特性
- Synonyms: goat anti-Glycerol Kinase Antibody HRP Conjugation, Peroxidase Conjugated goat anti-Glycerol Kinase Antibody, ATP glycerol 3 phosphotransferase antibody, GK antibody, GK antibody, GK1 antibody, GKD antibody, Glycerokinase antibody, Glycerol kinase antibody, Glycerol kinase deficiency antibody
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纯化方法
- Glycerol Kinase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Glycerol Kinase [Cellulomonas species]
Immunogen Type: Native Protein
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anti-Glycerol Kinase (GK) (AA 1-524) antibody
GK 适用: 人 WB, IHC (p) 宿主: 小鼠 Polyclonal unconjugated
anti-Glycerol Kinase (GK) antibodyGK 适用: 人, 小鼠, 大鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Glycerol Kinase (GK) (AA 2-94) antibodyGK 适用: 人 ELISA, WB 宿主: 小鼠 Polyclonal unconjugated
anti-Glycerol Kinase (GK) (AA 1-524) antibodyGK 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Glycerol Kinase (GK) antibodyGK 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glycerol Kinase (GK) (AA 1-180) antibodyGK 适用: 人, 小鼠, 大鼠 ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Glycerol Kinase (GK) (AA 21-120) antibodyGK 适用: 人 ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Glycerol Kinase (GK) (AA 2-502) antibodyGK 适用: E. coli ELISA, WB 宿主: 兔 Polyclonal unconjugated
anti-Glycerol Kinase (GK) (AA 2-502) antibody (Biotin)GK 适用: E. coli ELISA, WB 宿主: 兔 Polyclonal Biotin
anti-Glycerol Kinase (GK) (AA 461-510) antibodyGK 适用: 人, 小鼠, 大鼠 ELISA, WB, IHC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
- Application Note: Anti-Glycerol Kinase has been tested by ELISA and western blot. this product is assayed against 1.0 μg of Glycerol Kinase [Cellulomonas species] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:2,500 of the reconstitution concentration is suggested for this product. Western Blot Dilution: 1:500 - 1:2,500 ELISA Dilution: 1:1,000 - 1:5,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- Glycerol Kinase (GK)
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别名
- glpK
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背景
- Background: Glycerol Kinase is a key enzyme in the regulation of glycerol uptake and metabolism. Glycerol kinase is a phosphotransferase enzyme involved in triglycerides and glycerophospholipids synthesis.
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基因ID
- 6960048
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UniProt
- D0VZG4
抗原
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