GlnA 抗体 (HRP)
适用: Microbial
WB, ELISA, IP
宿主: 山羊
Polyclonal
HRP
-
- 抗原
- GlnA
-
适用
- Microbial
-
宿主
- 山羊
-
克隆类型
- 多克隆
-
标记
- HRP
- 应用范围
- Western Blotting (WB), ELISA, Immunoprecipitation (IP)
- 原理
- Glutamine Synthetase Antibody Peroxidase Conjugated
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Glutamine Synthetase [Microbial].
- 产品特性
- Synonyms: goat anti-Glutamine Synthetase Antibody HRP Conjugation, Peroxidase Conjugated goat anti-Glutamine Synthetase Antibody, Glutamine synthetase
- 纯化方法
- Glutamine Synthetase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- 过滤
- Sterile filtered
- 免疫原
-
Immunogen: Glutamine Synthetase [Microbial]
Immunogen Type: Native Protein
-
anti-GlnA antibody
适用: Synechococcus PCC 7942, Synechocystis PCC 6803 WB 宿主: 小鸡 Polyclonal unconjugated
-
- 应用备注
- Application Note: Anti-Glutamine Synthetase has been assayed against 1.0 μg of Glutamine Synthetase [Microbial] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 of the reconstitution concentration is suggested for this product. Western Blot Dilution: 1:500 - 1:2,500 Immunoprecipitation Dilution: 1:100 ELISA Dilution: 1:2,000 - 1:10,000 Other: User Optimized
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
-
- 抗原
- GlnA
- 背景
- Background: Glutamine Synthetase catalyzes the ATP-dependent condensation of glutamate with ammonia to yield glutamine. Glutamine Synthetase is present predominantly in the brain, kidneys, and liver. In the brain it participates in the metabolic regulation of glutamate, the detoxification of brain ammonia, the assimilation of ammonia, recyclization of neurotransmitters, and termination of neurotransmitter signals. Glutamine Synthetase, in the brain, is found primarily is astrocytes.
- 基因ID
- 3345165
- UniProt
- Q79VE3
-
You are here:
