HAO1 抗体 (HRP)
Quick Overview for HAO1 抗体 (HRP) (ABIN7565881)
抗原
See all HAO1 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Glucose Oxidase Antibody Peroxidase Conjugated
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交叉反应 (详细)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Glucose Oxidase [Aspergillus niger].
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产品特性
- Synonyms: rabbit anti-Glucose Oxidase Antibody Peroxidase Conjugation, HRP Conjugated rabbit anti-Glucose Oxidase Antibody, Beta D Glucose Oxygen 1 Oxido Reductase antibody, Glucose oxidase (Precursor) antibody, Glucose Oxyhydrase antibody, GOD antibody
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纯化方法
- Glucose Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Glucose Oxidase [Aspergillus niger]
Immunogen Type: Native Protein
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应用备注
- Application Note: Anti-Glucose Oxidase Peroxidase Conjugated Antibody has been tested by ELISA and western blot and is useful in Immunohistochemistry. Optimal titers should be obtained by researcher. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:1,000 - 1:5,000 ELISA Dilution: 1:5,000 - 1:25,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- HAO1 (Hydroxyacid Oxidase (Glycolate Oxidase) 1 (HAO1))
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别名
- gox
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背景
- Background: Glucose Oxidase is an oxido-reductase that catalyzes the oxidation of glucose to hydrogen peroxide and D-glucono-δ-lactone. In cells, it aids in breaking the sugar down into its metabolites, used for the determination of free glucose in body fluids, and has many applications in biotechnologies.
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UniProt
- P13006
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途径
- Monocarboxylic Acid Catabolic Process
抗原
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